Endocytosis of the glutamate receptor subunit GluK3 controls polarized trafficking
Journal of Neuroscience. 2011-08-10; 31(32): 11645-11654
DOI: 10.1523/JNEUROSCI.2206-11.2011
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1. J Neurosci. 2011 Aug 10;31(32):11645-54. doi: 10.1523/JNEUROSCI.2206-11.2011.
Endocytosis of the glutamate receptor subunit GluK3 controls polarized
trafficking.
Huyghe D(1), Veran J, Labrousse VF, Perrais D, Mulle C, Coussen F.
Author information:
(1)University of Bordeaux, Interdisciplinary Institute for Neuroscience, and CNRS
UMR 5297, F-33000 Bordeaux, France.
Erratum in
J Neurosci. 2011 Oct 5;31(40):14450.
Kainate receptors (KARs) are widely expressed in the brain and are present at
both presynaptic and postsynaptic sites. GluK3-containing KARs are thought to
compose presynaptic autoreceptors that facilitate hippocampal mossy fiber
synaptic transmission. Here we identify molecular mechanisms that underlie the
polarized trafficking of KARs composed of the GluK3b splice variant. Endocytosis
followed by degradation is driven by a dileucine motif on the cytoplasmic
C-terminal domain of GluK3b in heterologous cells, in cultured hippocampal
neurons, and in dentate granule cells from organotypic slice cultures. The
internalization of GluK3b is clathrin and dynamin2 dependent. GluK3b is
differentially endocytosed in dendrites as compared to the axons. These data
suggest that the polarized trafficking of KARs in neurons could be controlled by
the regulation of receptor endocytosis.
DOI: 10.1523/JNEUROSCI.2206-11.2011
PMID: 21832194 [Indexed for MEDLINE]