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Séminaire impromptu – Damien Jullié

lundi 4 mars 2019 / 11:30

Lieu : Centre Broca Nouvelle-Aquitaine, conference room


Title: « A discrete presynaptic vesicle cycle for neuromodulator receptors »


Damien Jullié
Damien Jullié

Damien Jullié, PhD
Post-doctoral fellow,
Department of Cellular and Molecular Pharmacology and Department of Psychiatry,
University of California, San Francisco School of Medicine

Invité par: David Perrais
IINS

Abstract

A major function of GPCRs is to inhibit neurotransmitter release from presynaptic terminals. This requires ligand-activated receptors to couple locally to effectors at terminals. The current understanding of how this is achieved is based on receptor immobilization on the presynaptic surface. Here we show that opioid peptide receptors, GPCRs which mediate highly sensitive presynaptic inhibition, are instead dynamic in axons. Opioid receptors diffuse rapidly throughout the axon surface and internalize after ligand-induced activation specifically at presynaptic terminals. We delineate a parallel regulated endocytic cycle for GPCRs at the presynapse, separate from the synaptic vesicle cycle, which clears activated receptors from the presynaptic surface and mediates local reinsertion to maintain the diffusible surface pool. We propose an alternate strategy to achieve local control of presynaptic effectors that, opposite to being based on receptor immobilization, is based on lateral mobility of receptors and leverages the inherently allosteric nature of GPCR – effector coupling.

 

Selected publications

Stoeber M, Jullié D, Lobingier BT, Laeremans T, Steyaert J, Schiller PW, Manglik A, von Zastrow M. A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action. Neuron. 2018 Jun 6;98(5):963-976.

Eichel K, Jullié D, Barsi-Rhyne B, Latorraca NR, Masureel M, Sibarita JB, Dror RO, von Zastrow M. Catalytic activation of β-arrestin by GPCRs. Nature. 2018 May;557(7705):381-386.

Rosendale M, Jullié D, Choquet D, Perrais D. Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation. Cell Rep. 2017 Feb 21;18(8):1840-1847.

Jullié D, Choquet D, Perrais D. Recycling endosomes undergo rapid closure of a fusion pore on exocytosis in neuronal dendrites. J Neurosci. 2014 Aug 13;34(33):11106-18.

 

Prochainement

  1. Séminaire – Suzana Herculano-Houzel

    vendredi 26 avril 2019 / 11:30

    Invitant : Thomas Boraud

  2. Séminaire impromptu – Maxime Assous

    vendredi 3 mai 2019 / 10:00 - 11:30

    Extrinsic innervation of striatal interneurons and implications for the local microcircuitry.

  3. Conférence mensuelle – Richard Robitaille

    vendredi 3 mai 2019 / 11:30 - 12:30

    Regulation of synaptic structure ad function by glial celles in health and disease

  4. Séminaire – Stéphanie Trouche

    vendredi 10 mai 2019 / 11:30 - 12:30

    Invitant : Catherine Le Moine

  5. Journée Bordeaux Neurocampus

    mercredi 15 mai 2019 / 09:00 - 18:00

    (Haut Carré, Talence)

Détails

Date :
lundi 4 mars 2019
Heure :
11:30
Catégorie d’Évènement: