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Séminaire impromptu – Damien Jullié

lundi 4 mars 2019 / 11:30

Lieu : Centre Broca Nouvelle-Aquitaine, conference room


Title: « A discrete presynaptic vesicle cycle for neuromodulator receptors »


Damien Jullié
Damien Jullié

Damien Jullié, PhD
Post-doctoral fellow,
Department of Cellular and Molecular Pharmacology and Department of Psychiatry,
University of California, San Francisco School of Medicine

Invité par: David Perrais
IINS

Abstract

A major function of GPCRs is to inhibit neurotransmitter release from presynaptic terminals. This requires ligand-activated receptors to couple locally to effectors at terminals. The current understanding of how this is achieved is based on receptor immobilization on the presynaptic surface. Here we show that opioid peptide receptors, GPCRs which mediate highly sensitive presynaptic inhibition, are instead dynamic in axons. Opioid receptors diffuse rapidly throughout the axon surface and internalize after ligand-induced activation specifically at presynaptic terminals. We delineate a parallel regulated endocytic cycle for GPCRs at the presynapse, separate from the synaptic vesicle cycle, which clears activated receptors from the presynaptic surface and mediates local reinsertion to maintain the diffusible surface pool. We propose an alternate strategy to achieve local control of presynaptic effectors that, opposite to being based on receptor immobilization, is based on lateral mobility of receptors and leverages the inherently allosteric nature of GPCR – effector coupling.

 

Selected publications

Stoeber M, Jullié D, Lobingier BT, Laeremans T, Steyaert J, Schiller PW, Manglik A, von Zastrow M. A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action. Neuron. 2018 Jun 6;98(5):963-976.

Eichel K, Jullié D, Barsi-Rhyne B, Latorraca NR, Masureel M, Sibarita JB, Dror RO, von Zastrow M. Catalytic activation of β-arrestin by GPCRs. Nature. 2018 May;557(7705):381-386.

Rosendale M, Jullié D, Choquet D, Perrais D. Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation. Cell Rep. 2017 Feb 21;18(8):1840-1847.

Jullié D, Choquet D, Perrais D. Recycling endosomes undergo rapid closure of a fusion pore on exocytosis in neuronal dendrites. J Neurosci. 2014 Aug 13;34(33):11106-18.

 

Prochainement

  1. Cajal course : Biosensors and actuators for cellular and systems neuroscience

    mardi 25 juin 2019 - mardi 9 juillet 2019

    Les séminaires sont ouverts à tous.

  2. Conférence – Stuart Firestein

    jeudi 27 juin 2019 / 14:00

    How Biology Perceives Chemistry: The Mammalian Olfactory System

  3. Conférence Labex Trail – Goran Angelovski

    vendredi 28 juin 2019 / 14:30

    Molecular fMRI with Bioresponsive Probes

  4. Atout neurones

    samedi 29 juin 2019 / 14:00 - 20:00

    Tournoi de bridge au profit de la recherche sur le cerveau et conférence d'Olivier Nicole (IMN)

  5. Ramène (pas) ta science ! à la kermesse des sciences

    dimanche 30 juin 2019 / 14:00 - 23:00

    Parc Peixotto, Talence

Détails

Date :
lundi 4 mars 2019
Heure :
11:30
Catégorie d’Évènement: