Loading Events

« All Events

  • This event has passed.

PHD seminar – Stéphane Vassilopoulos

14 January 2018 / 11:30 - 12:30

Metal-replica EM in the 21st century

14 juin 2018 à 11:30 (Amphi Broca Nouvelle Aquitaine / Campus Carreire)

Stéphane Vassilopoulos
Myologie Centre de Recherche/Paris
Institut de Myologie UMRS 974 UPMC-Inserm, FRE 3617 CNRS

Invitant : David Perrais
PhD, IINS , team: Nano-scale organization, dynamics of synaptic proteins and membrane trafficking.

Abstract :

In cell biology, particular importance is given to developing new methods of sample preparation that will achieve a more natural appearance of samples in the microscope. To accomplish this, scientists have developed the metal-replica electron microscopy (EM) technique directly on unroofed cultured cells. This particular EM technique permits visualization of the inner side of cell membranes and their components. Metal-replicas have profoundly shaped our understanding of the functional morphology of the cell and used to capture several different cellular processes including remodeling of cytoskeletons, membrane trafficking, cell-to-cell contacts and formation of extracellular matrices. Combined to transmission electron microscopy (TEM), it is an invaluable method to reveal the composition of the PM and to directly observe macromolecular complexes including the cytoskeleton and endocytic membrane invaginations at high resolution. Here, I will outline the history of this particular EM technique and describe different methodologies and some key applications in cell biology including visualization of the cytoskeleton and different endocytic structures such as clathrin coated pits and caveolae.

Selected publications

Franck, A., Laine, J., Moulay, G., Trichet, M., Gentil, C., Fongy, A., Bigot, A., Benkhelifa-Ziyyat, S., Lacene, E., Thao Bui, M., et al. (2018). Clathrin plaques form mechanotransducing platforms. bioRxiv.

Randrianarison-Huetz, V., Papaefthymiou, A., Herledan, G., Noviello, C., Faradova, U., Collard, L., Pincini, A., Schol, E., Decaux, J.F., Maire, P., et al. (2018). Srf controls satellite cell fusion through the maintenance of actin architecture. J. Cell Biol. 217, 685–700.

Elkhatib, N., Bresteau, E., Baschieri, F., Rioja, A.L., van Niel, G., Vassilopoulos, S., and Montagnac, G. (2017). Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science 356.

Lamaze, C., Tardif, N., Dewulf, M., Vassilopoulos, S., and Blouin, C.M. (2017). The caveolae dress code: structure and signaling. Curr. Opin. Cell Biol. 47, 117–125.

Vassilopoulos, S., Gentil, C., Lainé, J., Buclez, P.-O., Franck, A., Ferry, A., Précigout, G., Roth, R., Heuser, J.E., Brodsky, F.M., et al. (2014). Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J. Cell Biol. 205, 377–393.

Coming soon

  1. Cajal course : Biosensors and actuators for cellular and systems neuroscience

    Tuesday 25 June 2019 - Tuesday 9 July 2019

    Talks are open to everyone

  2. Conference Labex Trail – Goran Angelovski

    Friday 28 June 2019 / 14:30

    Molecular fMRI with Bioresponsive Probes

  3. Atout neurones

    Saturday 29 June 2019 / 14:00 - 20:00

    Tournoi de bridge au profit de la recherche sur le cerveau et conférence d'Olivier Nicole (IMN)

  4. Seminar – Anne Brunet

    Tuesday 2 July 2019 / 11:30

    Understanding and modeling aging

  5. Seminar – Stephanie Borgland

    Friday 5 July 2019 / 11:00 - 12:00

    Diet induced plasticity in the orbitofrontal cortex: implications for overeating


14 January 2018
11:30 - 12:30