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Séminaire – Stéphane Vassilopoulos

14 janvier 2018 / 11:30 - 12:30

Metal-replica EM in the 21st century

14 juin 2018 à 11:30 (Amphi Broca Nouvelle Aquitaine / Campus Carreire)

Stéphane Vassilopoulos
Myologie Centre de Recherche/Paris
Institut de Myologie UMRS 974 UPMC-Inserm, FRE 3617 CNRS

Invitant : David Perrais
PhD, IINS , team: Nano-scale organization, dynamics of synaptic proteins and membrane trafficking.

Abstract :

In cell biology, particular importance is given to developing new methods of sample preparation that will achieve a more natural appearance of samples in the microscope. To accomplish this, scientists have developed the metal-replica electron microscopy (EM) technique directly on unroofed cultured cells. This particular EM technique permits visualization of the inner side of cell membranes and their components. Metal-replicas have profoundly shaped our understanding of the functional morphology of the cell and used to capture several different cellular processes including remodeling of cytoskeletons, membrane trafficking, cell-to-cell contacts and formation of extracellular matrices. Combined to transmission electron microscopy (TEM), it is an invaluable method to reveal the composition of the PM and to directly observe macromolecular complexes including the cytoskeleton and endocytic membrane invaginations at high resolution. Here, I will outline the history of this particular EM technique and describe different methodologies and some key applications in cell biology including visualization of the cytoskeleton and different endocytic structures such as clathrin coated pits and caveolae.

Selected publications

Franck, A., Laine, J., Moulay, G., Trichet, M., Gentil, C., Fongy, A., Bigot, A., Benkhelifa-Ziyyat, S., Lacene, E., Thao Bui, M., et al. (2018). Clathrin plaques form mechanotransducing platforms. bioRxiv.

Randrianarison-Huetz, V., Papaefthymiou, A., Herledan, G., Noviello, C., Faradova, U., Collard, L., Pincini, A., Schol, E., Decaux, J.F., Maire, P., et al. (2018). Srf controls satellite cell fusion through the maintenance of actin architecture. J. Cell Biol. 217, 685–700.

Elkhatib, N., Bresteau, E., Baschieri, F., Rioja, A.L., van Niel, G., Vassilopoulos, S., and Montagnac, G. (2017). Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science 356.

Lamaze, C., Tardif, N., Dewulf, M., Vassilopoulos, S., and Blouin, C.M. (2017). The caveolae dress code: structure and signaling. Curr. Opin. Cell Biol. 47, 117–125.

Vassilopoulos, S., Gentil, C., Lainé, J., Buclez, P.-O., Franck, A., Ferry, A., Précigout, G., Roth, R., Heuser, J.E., Brodsky, F.M., et al. (2014). Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J. Cell Biol. 205, 377–393.

Prochainement

  1. Séminaire – Suzana Herculano-Houzel

    vendredi 26 avril 2019 / 11:30

    Life slows down with more neurons in the cerebral cortex

  2. XIIIth IBAGS Meeting

    dimanche 28 avril 2019 - jeudi 2 mai 2019

    The XIIIth edition of the IBAGS meeting will be held in Biarritz from the 28th of April to the 2nd of May. In this meeting, we gather outstanding scientists and clinicians from all over the world to discuss about topics related to basal ganglia pathways, physiology, reinforcement learning and related disorders. Tutorials on basic science and clinical aspects of the basal ganglia will be given on the introducing day of the meeting. http://www.ibags2019.com/

  3. Séminaire impromptu – Maxime Assous

    vendredi 3 mai 2019 / 10:00 - 11:30

    Extrinsic innervation of striatal interneurons and implications for the local microcircuitry.

  4. Conférence mensuelle – Richard Robitaille

    vendredi 3 mai 2019 / 11:30 - 12:30

    Regulation of synaptic structure ad function by glial cells in health and disease

  5. Séminaire – Stéphanie Trouche

    vendredi 10 mai 2019 / 11:30 - 12:30

    Invitant : Catherine Le Moine

Détails

Date :
14 janvier 2018
Heure :
11:30 - 12:30