Synaptic Control of Secretory Trafficking in Dendrites

Cyril Hanus, Lisa Kochen, Susanne tom Dieck, Victor Racine, Jean-Baptiste Sibarita, Erin M. Schuman, Michael D. Ehlers
Cell Reports. 2014-06-01; 7(6): 1771-1778
DOI: 10.1016/j.celrep.2014.05.028

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1. Cell Rep. 2014 Jun 26;7(6):1771-8. doi: 10.1016/j.celrep.2014.05.028. Epub 2014
Jun 12.

Synaptic control of secretory trafficking in dendrites.

Hanus C(1), Kochen L(2), Tom Dieck S(2), Racine V(3), Sibarita JB(4), Schuman
EM(2), Ehlers MD(5).

Author information:
(1)Max Planck Institute for Brain Research, Frankfurt 60438, Germany. Electronic
address: .
(2)Max Planck Institute for Brain Research, Frankfurt 60438, Germany.
(3)Institute of Molecular & Cell Biology, Agency for Science, Technology and
Research, Singapore 138673, Singapore.
(4)Interdisciplinary Institute for Neuroscience, Bordeaux 33077, France.
(5)Neuroscience Research Unit, Pfizer Worldwide Research and Development,
Cambridge, MA 02139, USA. Electronic address: .

Localized signaling in neuronal dendrites requires tight spatial control of
membrane composition. Upon initial synthesis, nascent secretory cargo in
dendrites exits the endoplasmic reticulum (ER) from local zones of ER complexity
that are spatially coupled to post-ER compartments. Although newly synthesized
membrane proteins can be processed locally, the mechanisms that control the
spatial range of secretory cargo transport in dendritic segments are unknown.
Here, we monitored the dynamics of nascent membrane proteins in dendritic post-ER
compartments under regimes of low or increased neuronal activity. In response to
activity blockade, post-ER carriers are highly mobile and are transported over
long distances. Conversely, increasing synaptic activity dramatically restricts
the spatial scale of post-ER trafficking along dendrites. This activity-induced
confinement of secretory cargo requires site-specific phosphorylation of the
kinesin motor KIF17 by Ca(2+)/calmodulin-dependent protein kinases (CaMK). Thus,
the length scales of early secretory trafficking in dendrites are tuned by
activity-dependent regulation of microtubule-dependent transport.

Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

DOI: 10.1016/j.celrep.2014.05.028
PMCID: PMC5321479
PMID: 24931613 [Indexed for MEDLINE]

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