Reprogramming cell shape with laser nano-patterning.

T. Vignaud, R. Galland, Q. Tseng, L. Blanchoin, J. Colombelli, M. Thery
Journal of Cell Science. 2012-02-22; 125(9): 2134-2140
DOI: 10.1242/jcs.104901

Read on PubMed

1. J Cell Sci. 2012 May 1;125(Pt 9):2134-40. doi: 10.1242/jcs.104901. Epub 2012 Feb

Reprogramming cell shape with laser nano-patterning.

Vignaud T(1), Galland R, Tseng Q, Blanchoin L, Colombelli J, Théry M.

Author information:
(1)Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherche en
Technologies et Sciences pour le Vivant, CNRS/UJF/INRA/CEA, Grenoble, France.

Cell shape in vitro can be directed by geometrically defined micropatterned
adhesion substrates. However conventional methods are limited by the fixed
micropattern design, which cannot recapitulate the dynamic changes of the cell
microenvironment. Here, we manipulate the shape of living cells in real time by
using a tightly focused pulsed laser to introduce additional geometrically
defined adhesion sites. The sub-micrometer resolution of the laser patterning
allowed us to identify the critical distances between cell adhesion sites
required for cell shape extension and contraction. This easy-to-handle method
allows the precise control of specific actin-based structures that regulate cell
architecture. Actin filament bundles or branched meshworks were induced,
displaced or removed in response to specific dynamic modifications of the cell
adhesion pattern. Isotropic branched actin meshworks could be forced to assemble
new stress fibers locally and polarised in response to specific geometrical cues.

DOI: 10.1242/jcs.104901
PMID: 22357956 [Indexed for MEDLINE]

Know more about