NMDA receptor surface mobility depends on NR2A-2B subunits

L. Groc, M. Heine, S. L. Cousins, F. A. Stephenson, B. Lounis, L. Cognet, D. Choquet
Proceedings of the National Academy of Sciences. 2006-11-21; 103(49): 18769-18774
DOI: 10.1073/pnas.0605238103

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1. Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18769-74. Epub 2006 Nov 21.

NMDA receptor surface mobility depends on NR2A-2B subunits.

Groc L(1), Heine M, Cousins SL, Stephenson FA, Lounis B, Cognet L, Choquet D.

Author information:
(1)Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche (UMR) 5091,
Centre National de la Recherche Scientifique (CNRS), Université Bordeaux 2, 33077
Bordeaux, France.

The NR2 subunit composition of NMDA receptors (NMDARs) varies during development,
and this change is important in NMDAR-dependent signaling. In particular,
synaptic NMDAR switch from containing mostly NR2B subunit to a mixture of NR2B
and NR2A subunits. The pathways by which neurons differentially traffic NR2A- and
NR2B-containing NMDARs are poorly understood. Using single-particle and -molecule
approaches and specific antibodies directed against NR2A and NR2B extracellular
epitopes, we investigated the surface mobility of native NR2A and NR2B subunits
at the surface of cultured neurons. The surface mobility of NMDARs depends on the
NR2 subunit subtype, with NR2A-containing NMDARs being more stable than
NR2B-containing ones, and NR2A subunit overexpression stabilizes surface
NR2B-containing NMDARs. The developmental change in the synaptic surface content
of NR2A and NR2B subunits was correlated with a developmental change in the time
spent by the subunits within synapses. This suggests that the switch in synaptic
NMDAR subtypes depends on the regulation of the receptor surface trafficking.

DOI: 10.1073/pnas.0605238103
PMCID: PMC1693737
PMID: 17124177 [Indexed for MEDLINE]

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