Mass spectrometry imaging and the ‘third wave’ of anatomical neuropathology
Lieu : CGFB (séminaire annulé)
While biologists view proteins, lipids, carbohydrates and nucleic acids as the building blocks of life, the chemical elements represent the fundamental constituents of a living organism. Cell types, biological functions and even disease processes have unique elemental signatures. In cases where minor and trace elements are directly implicated in the mechanism of a disease this signature can be stark, while other scenarios can arise where subtler changes are apparent. The emerging potential as a tool for clinical pathology will be discussed, highlighting how concurrent advances in technology, image analysis, machine learning, and a growing appreciation that the chemical elements are not regulated in isolation are converging to position elemental imaging as a valuable resource for digital pathology and the decision-making process in treating neurological disorders.
S. Genoud, B. Roberts, A. Gunn, G. Halliday, S. Lewis, H. Ball, D. Hare, K. Double. Subcellular compartmentalisation of copper, iron, manganese, and zinc in the Parkinson’s disease brain. Metallomics, (2017), 9, 1447-1455.
B. Trist, K. Davies, V. Cottam, S. Genoud, R. Ortega, S. Roudeau, A. Carmona, K. De Silva, V. Wagner, S. Lewis, P. Sachdev, B. Smith, C. Troakes, C. Vance, C. Shaw, S. Al-Sarraj, H. Ball, G. Halliday, D. Hare, K. Double. Amyotrophic lateral sclerosis-like superoxide dismutase 1 proteinopathy is associated with neuronal loss in Parkinson’s disease brain. Acta Neuropathologica, (2017), 134, 113-127.
D. Hare, E. Raven, B. Roberts, M. Bogeski, S. Portbury, C. McLean, C. Masters, J. Connor, A. Bush, P. Crouch, P. Doble. Laser ablation-inductively coupled plasma-mass spectrometry imaging of white and gray matter iron distribution in Alzheimer’s disease
frontal cortex. NeuroImage, (2016), 137, 124-134.
B. Paul, D. Hare, D. Bishop, C. Paton, V. Nguyen, N. Cole, M. Niedzwiecki, E. Andreozzi, A. Vais, J. Billings, L. Bray, A. Bush, G. McColl, B. Roberts, P. Adlard, D. Finkelstein, J. Hellstrom, J. Hergt, J. Woodhead, P. Doble*. Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging. Chemical Science, (2015), 6, 5383-5393.
D. Hare, J. Doecke, N. Faux, A. Rembach, I. Volitakis, C. Fowler, R. Grimm, P. Doble, R. Cherny, C. Masters, A. Bush, B. Roberts. Decreased plasma iron in Alzheimer’s disease is due to transferrin desaturation. ACS Chemical Neuroscience (2015) 6, 398-402.