Cell adhesion molecules in synapse assembly
Our team aims at characterizing the molecular mechanisms of synapse formation, with a focus on the function of cell adhesion molecules including neurexins and neuroligins. We are studying the membrane dynamics and nanoscale organization of these adhesion molecules at synapses, and their contribution to the recruitment of scaffolding proteins and glutamate receptors, by combining single molecule detection and computer simulations. We are also characterizing a neuroligin-1 phosphotyrosine signaling mechanism regulating the differential assembly of excitatory versus inhibitory synapses, by using a combination of electrophysiology and optogenetic approaches. Finally, we are investigating the trafficking and function of neurexins and neuroligins harboring genetic mutations identified in autism spectrum disorders.
Innovative techniques: Single molecule dynamics, computer simulations, micropatterned substrates, single molecule pull-down, protein labeling with monomeric ligands, optogenetic induction of phosphotyrosine signaling, single cell electroporation, dual patch-clamp.
Mots clés
Adhésion cellulaire et cytosquelette, Bio-informatique et analyse des données, Imagerie cellulaire in vitro ou in vivo, Microscopie super-résolutive, Optogénétique et chemogénétique, Physiologie synaptique, Signalisation, Synapse molecular complexesSelected publications
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miR ‐124‐dependent tagging of synapses by synaptopodin enables input‐specific homeostatic plasticity
Sandra Dubes, Anaïs Soula, Sébastien Benquet, Béatrice Tessier, Christel Poujol, Alexandre Favereaux, Olivier Thoumine, Mathieu Letellier.
The EMBO Journal. 2022-07-25.
DOI: 10.15252/embj.2021109012 -
MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior.
Andrea Toledo, Mathieu Letellier, Giorgia Bimbi, Béatrice Tessier, Sophie Daburon, Alexandre Favereaux, Ingrid Chamma, Kristel Vennekens, Jeroen Vanderlinden, Matthieu Sainlos, Joris de Wit, Daniel Choquet, Olivier Thoumine.
eLife. 2022-05-09. 11
DOI: 10.7554/elife.75233 -
High-Resolution Fluorescence Imaging Combined With Computer Simulations to Quantitate Surface Dynamics and Nanoscale Organization of Neuroligin-1 at Synapses.
Matthieu Lagardère, Adèle Drouet, Matthieu Sainlos, Olivier Thoumine.
Front. Synaptic Neurosci.. 2022-04-25. 14
DOI: 10.3389/fnsyn.2022.835427 -
Role of regulatory C‐terminal motifs in synaptic confinement of LRRTM2
Konstantina Liouta, Julia Chabbert, Sebastien Benquet, Béatrice Tessier, Vincent Studer, Matthieu Sainlos, Joris De Wit, Olivier Thoumine, Ingrid Chamma.
Biol. Cell. 2021-09-27.
DOI: 10.1111/boc.202100026 -
FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.
Matthieu Lagardère, Ingrid Chamma, Emmanuel Bouilhol, Macha Nikolski, Olivier Thoumine.
Sci Rep. 2020-11-17. 10 (1)
DOI: 10.1038/s41598-020-75814-y -
Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation
Mathieu Letellier, Matthieu Lagardère, Béatrice Tessier, Harald Janovjak, Olivier Thoumine.
eLife. 2020-04-23. 9
DOI: 10.7554/elife.52027 -
Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth
Steve Dos-Santos Carvalho, Maite M Moreau, Yeri Esther Hien, Michael Garcia, Nathalie Aubailly, Deborah J Henderson, Vincent Studer, Nathalie Sans, Olivier Thoumine, Mireille Montcouquiol.
eLife. 2020-01-07. 9
DOI: 10.7554/eLife.51822 -
miRNA-Dependent Control of Homeostatic Plasticity in Neurons
Sandra Dubes, Alexandre Favereaux, Olivier Thoumine, Mathieu Letellier.
Front. Cell. Neurosci.. 2019-12-05. 13
DOI: 10.3389/fncel.2019.00536 -
A super-resolution platform for correlative live single-molecule imaging and STED microscopy
V. V. G. Krishna Inavalli, Martin O. Lenz, Corey Butler, Julie Angibaud, Benjamin Compans, Florian Levet, Jan Tønnesen, Olivier Rossier, Gregory Giannone, Olivier Thoumine, Eric Hosy, Daniel Choquet, Jean-Baptiste Sibarita, U. Valentin Nägerl.
Nat Methods. 2019-10-21.
DOI: 10.1038/s41592-019-0611-8 -
TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering
Edoardo Moretto, Anna Longatti, Luca Murru, Ingrid Chamma, Alessandro Sessa, Jonathan Zapata, Eric Hosy, Matthieu Sainlos, Julien Saint-Pol, Eric Rubinstein, Daniel Choquet, Vania Broccoli, Giampietro Schiavo, Olivier Thoumine, Maria Passafaro.
Cell Reports. 2019-10-01. 29 (5) : 1130-1146.e8.
DOI: 10.1016/j.celrep.2019.09.051 -
A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation.
Aurora Pignata, Hugo Ducuing, Leila Boubakar, Thibault Gardette, Karine Kindbeiter, Muriel Bozon, Servane Tauszig-Delamasure, Julien Falk, Olivier Thoumine, Valérie Castellani.
Cell Reports. 2019-10-01. 29 (2) : 347-362.e5.
DOI: 10.1016/j.celrep.2019.08.098 -
Differential role of pre- and postsynaptic neurons in the activity-dependent control of synaptic strengths across dendrites
Mathieu Letellier, Florian Levet, Olivier Thoumine, Yukiko Goda.
PLoS Biol. 2019-06-05. 17 (6) : e2006223.
DOI: 10.1371/journal.pbio.2006223 -
Small RNA-Seq reveals novel miRNAs shaping the transcriptomic identity of rat brain structures
Anaïs Soula, Mélissa Valere, María-José López-González, Vicky Ury-Thiery, Alexis Groppi, Marc Landry, Macha Nikolski, Alexandre Favereaux.
Life Sci. Alliance. 2018-10-01. 1 (5) : e201800018.
DOI: 10.26508/lsa.201800018 -
A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation
Mathieu Letellier, Zsófia Szíber, Ingrid Chamma, Camille Saphy, Ioanna Papasideri, Béatrice Tessier, Matthieu Sainlos, Katalin Czöndör, Olivier Thoumine.
Nat Commun. 2018-09-28. 9 (1)
DOI: 10.1038/s41467-018-06220-2 -
Pre-post synaptic alignment through neuroligin-1 tunes synaptic transmission efficiency
Kalina T Haas, Benjamin Compans, Mathieu Letellier, Thomas M Bartol, Dolors Grillo-Bosch, Terrence J Sejnowski, Matthieu Sainlos, Daniel Choquet, Olivier Thoumine, Eric Hosy.
eLife. 2018-07-25. 7
DOI: 10.7554/eLife.31755 -
Spinal miRNA-124 regulates synaptopodin and nociception in an animal model of bone cancer pain.
Sara Elramah, María José López-González, Matthieu Bastide, Florence Dixmérias, Olivier Roca-Lapirot, Anne-Cécile Wielanek-Bachelet, Anne Vital, Thierry Leste-Lasserre, Alexandre Brochard, Marc Landry, Alexandre Favereaux.
Sci Rep. 2017-09-08. 7 (1)
DOI: 10.1038/s41598-017-10224-1 -
A Triad of Crystals Sheds Light on MDGA Interference with Neuroligation.
Olivier Thoumine, Pascale Marchot.
Neuron. 2017-08-01. 95 (4) : 729-732.
DOI: 10.1016/j.neuron.2017.08.001 -
Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin
Ingrid Chamma, Olivier Rossier, Grégory Giannone, Olivier Thoumine, Matthieu Sainlos.
Nat Protoc. 2017-03-09. 12 (4) : 748-763.
DOI: 10.1038/nprot.2017.010 -
Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin.
Ingrid Chamma, Mathieu Letellier, Corey Butler, Béatrice Tessier, Kok-Hong Lim, Isabel Gauthereau, Daniel Choquet, Jean-Baptiste Sibarita, Sheldon Park, Matthieu Sainlos, Olivier Thoumine.
Nat Comms. 2016-03-16. 7 : 10773.
DOI: 10.1038/ncomms10773 -
Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones
Mikael Garcia, Cécile Leduc, Matthieu Lagardère, Amélie Argento, Jean-Baptiste Sibarita, Olivier Thoumine.
Proc Natl Acad Sci USA. 2015-05-18. 112 (22) : 6997-7002.
DOI: 10.1073/pnas.1423455112 -
miR-92a regulates expression of synaptic GluA1-containing AMPA receptors during homeostatic scaling.
Mathieu Letellier, Sara Elramah, Magali Mondin, Anaïs Soula, Andrew Penn, Daniel Choquet, Marc Landry, Olivier Thoumine, Alexandre Favereaux.
Nat Neurosci. 2014-07-13. 17 (8) : 1040-1042.
DOI: 10.1038/nn.3762
Team leader
Team member(s)
Chercheurs, Praticiens hospitaliers...
Alexandre Favereaux
Favereaux
(University Teacher- Researcher)
Mathieu Letellier Letellier (Researcher)
Méghane Sittewelle Sittewelle (Researcher Ingineer)
Mathieu Letellier Letellier (Researcher)
Méghane Sittewelle Sittewelle (Researcher Ingineer)
Ingénieur(e)s, technicien(ne)s
Béatrice Tessier
Tessier
Chloé Desquines Desquines
Benjamin Chauvineau Chauvineau
Tiffany Cloatre Cloatre
Lucie Franco Franco
Chloé Desquines Desquines
Benjamin Chauvineau Chauvineau
Tiffany Cloatre Cloatre
Lucie Franco Franco
Post-doctorant(s)
Doctorant(s)
Neuropsychologist(s) and speech therapist(s)
Ingénieur(s) hospitalier(s) et ARC