Cell adhesion molecules in synapse assembly
Our team aims at characterizing the molecular mechanisms of synapse formation, with a focus on the function of cell adhesion molecules including neurexins and neuroligins. We are studying the membrane dynamics and nanoscale organization of these adhesion molecules at synapses, and their contribution to the recruitment of scaffolding proteins and glutamate receptors, by combining single molecule detection and computer simulations. We are also characterizing a neuroligin-1 phosphotyrosine signaling mechanism regulating the differential assembly of excitatory versus inhibitory synapses, by using a combination of electrophysiology and optogenetic approaches. Finally, we are investigating the trafficking and function of neurexins and neuroligins harboring genetic mutations identified in autism spectrum disorders.
Innovative techniques: Single molecule dynamics, computer simulations, micropatterned substrates, single molecule pull-down, protein labeling with monomeric ligands, optogenetic induction of phosphotyrosine signaling, single cell electroporation, dual patch-clamp.
Mots clés
Adhésion cellulaire et cytosquelette, Bio-informatique et analyse des données, Complexes moléculaires de la synapse, Imagerie cellulaire in vitro ou in vivo, Microscopie super-résolutive, Optogénétique et chemogénétique, Physiologie synaptique, SignalisationSelected publications
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FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.
Matthieu Lagardère, Ingrid Chamma, Emmanuel Bouilhol, Macha Nikolski, Olivier Thoumine.
. 2020-02-06.
DOI: 10.1101/2020.02.06.937045 -
Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.
Steve Dos-Santos Carvalho, Maite M Moreau, Yeri Esther Hien, Michael Garcia, Nathalie Aubailly, Deborah J Henderson, Vincent Studer, Nathalie Sans, Olivier Thoumine, Mireille Montcouquiol.
eLife. 2020-01-07. 9
DOI: 10.7554/eLife.51822 -
miRNA-Dependent Control of Homeostatic Plasticity in Neurons.
Sandra Dubes, Alexandre Favereaux, Olivier Thoumine, Mathieu Letellier.
Front. Cell. Neurosci.. 2019-12-05. 13
DOI: 10.3389/fncel.2019.00536 -
Dynamics, nanoscale organization, and function of synaptic adhesion molecules.
Ingrid Chamma, Olivier Thoumine.
Molecular and Cellular Neuroscience. 2018-09-01. 91 : 95-107.
DOI: 10.1016/j.mcn.2018.04.007 -
Neurofibromatosis type 2 tumor suppressor protein is expressed in oligodendrocytes and regulates cell proliferation and process formation..
Andrea Toledo, Elena Grieger, Khalad Karram, Helen Morrison, Stephan L. Baader.
PLoS ONE. 2018-05-01. 13 (5) : e0196726.
DOI: 10.1371/journal.pone.0196726 -
Neuron-specific deletion of the Nf2 tumor suppressor impairs functional nerve regeneration.
Alexander Schulz, Robert Büttner, Andrea Toledo, Stephan L. Baader, Julia von Maltzahn, Andrey Irintchev, Reinhard Bauer, Helen Morrison.
PLoS ONE. 2016-07-28. 11 (7) : e0159718.
DOI: 10.1371/journal.pone.0159718 -
The Sorting Receptor SorCS1 Regulates Trafficking of Neurexin and AMPA Receptors.
Jeffrey N. Savas, Luís F. Ribeiro, Keimpe D. Wierda, Rebecca Wright, Laura A. DeNardo-Wilke, Heather C. Rice, Ingrid Chamma, Yi-Zhi Wang, Roland Zemla, Mathieu Lavallée-Adam, Kristel M. Vennekens, Matthew L. O’Sullivan, Joseph K. Antonios, Elizabeth A. Hall, Olivier Thoumine, Alan D. Attie, John R. Yates, Anirvan Ghosh, Joris de Wit.
Neuron. 2015-08-01. 87 (4) : 764-780.
DOI: 10.1016/j.neuron.2015.08.007 -
Structural characterization of a neuroblast-specific phosphorylated region of MARCKS.
Luzineide W. Tinoco, Jully L. Fraga, Cristiane D. AnoBom, Flavio R. Zolessi, Gonzalo Obal, Andrea Toledo, Otto Pritsch, Cristina Arruti.
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2014-04-01. 1844 (4) : 837-849.
DOI: 10.1016/j.bbapap.2014.02.016 -
Searching for novel Cdk5 substrates in brain by comparative phosphoproteomics of wild type and Cdk5-/- mice.
Erick Contreras-Vallejos, Elías Utreras, Daniel A. Bórquez, Michaela Prochazkova, Anita Terse, Howard Jaffe, Andrea Toledo, Cristina Arruti, Harish C. Pant, Ashok B. Kulkarni, Christian González-Billault.
PLoS ONE. 2014-03-21. 9 (3) : e90363.
DOI: 10.1371/journal.pone.0090363 -
Preferential localization of γh2AX foci in euchromatin of retina rod cells after DNA damage induction.
Laura Lafon-Hughes, María Vittoria Di Tomaso, Pablo Liddle, Andrea Toledo, Ana Laura Reyes-Ábalos, Gustavo A. Folle.
Chromosome Res. 2013-12-01. 21 (8) : 789-803.
DOI: 10.1007/s10577-013-9395-3 -
Activity-dependent regulation of the K/Cl transporter KCC2 membrane diffusion, clustering, and function in hippocampal neurons.
I. Chamma, M. Heubl, Q. Chevy, M. Renner, I. Moutkine, E. Eugene, J. C. Poncer, S. Levi.
Journal of Neuroscience. 2013-09-25. 33 (39) : 15488-15503.
DOI: 10.1523/JNEUROSCI.5889-12.2013 -
Role of the neuronal K-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission.
Ingrid Chamma, Quentin Chevy, Jean Christophe Poncer, Sabine Lévi.
Front. Cell. Neurosci.. 2012-01-01. 6
DOI: 10.3389/fncel.2012.00005 -
The neuronal K-Cl cotransporter KCC2 influences postsynaptic AMPA receptor content and lateral diffusion in dendritic spines.
G. Gauvain, I. Chamma, Q. Chevy, C. Cabezas, T. Irinopoulou, N. Bodrug, M. Carnaud, S. Levi, J. C. Poncer.
Proceedings of the National Academy of Sciences. 2011-08-30. 108 (37) : 15474-15479.
DOI: 10.1073/pnas.1107893108
Team member(s)
Chercheurs, Praticiens hospitaliers...
Mathieu Letellier Letellier (Researcher)
Ingénieur(e)s, technicien(ne)s
Post-doctorant(s)
Doctorant(s)