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Seminar – Julien Berro

23 November 2021 / 11:00 - 12:00


Force production and force sensing during clathrin-mediated endocytosis


Clathrin-mediated endocytosis (CME) consists of the formation of a vesicle out of a flat membrane in eukaryotic cells. When membrane tension and/or turgor pressure are high, actin dynamics is required to produce the forces required to invaginate the membrane and pinch it off into a vesicle. However, how the actin meshwork produces forces at the molecular level has remained elusive, because endocytic structures are transient, out of equilibrium, and diffraction limited. In this seminar, I will present results from mathematical modeling and experiments in yeast showing that actin polymerization alone is not sufficient force to invaginate the plasma membrane. I will also present new force production mechanisms by the actin meshwork that are not exclusively based on polymerization, and are relevant to other subcellular processes involving actin and membranes.

Selected publications

Lemière J, Ren Y, Berro J (2021) Rapid adaptation of endocytosis, exocytosis and eisosomes after an acute increase in membrane tension in yeast cells. eLife 10:e62084.

Ma R, Berro J (2021) Endocytosis against high turgor pressure is made easier by partial coating and freely rotating base. Biophysical Journal 120:1625–1640.

Martiel J-L, Michelot A, Boujemaa-Paterski R, Blanchoin L, Berro J (2020) Force Production by a Bundle of Growing Actin Filaments Is Limited by Its Mechanical Properties. Biophysical Journal 118:182–192.

Lacy MM, Baddeley D, Berro J (2019) Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis.  eLife 8:e52355.

Lacy MM, Ma R, Ravindra NG, Berro J (2018) Molecular mechanisms of force production in clathrin-mediated endocytosis. FEBS Letters 592:3586–3605.

Dr. Julien Berro
Associate Professor of Molecular Biophysics, Biochemistry, and Cell Biology
Yale University School of Medicine


Invited by

David Perrais

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23 November 2021
11:00 - 12:00
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