Optical techniques have become indispensable for biological research in recent decades. Systems neuroscience has seen some of the most exciting developments in this regard, following the development of genetically-encoded biosensors and actuators that allow multi-modal interrogation of neural circuit function. This course will provide participants with an in-depth understanding of the principles behind the design and application of these tools, and enable hands-on experience with optogenetic and chemogenetic actuators and with genetically encoded reporters of calcium, voltage and metabolism. Experimental work will span a wide range of systems and experimental preparations, utilizing standard microscopy methods as well as advanced hardware for parallel excitation and imaging of neuronal circuits. In addition to the practical aspects of utilizing these powerful tools, the course will cover the conceptual issues of data analysis and interpretation.
Weizmann Institute of Science
University of Bordeaux
This course is organised in partnership with the Bordeaux Imaging Center (BIC).
Haruhiko Bito – Tokyo University, Japan
Karl Deisseroth – Stanford University , USA
Stéphane Dieudonné – Inserm – Institut de Biologie de l’École Normale Supérieure (IBENS), France
Valentina Emiliani – Paris Descartes University, France
Oliver Griesbeck – MPI for Neurobiology , Germany
Peter Hegemann – Humboldt University , Germany
Thomas Kash – University of North Carolina , USA
Thomas Oertner – Hamburg Eppendorf University, Germany
Mark J. Schnitzer – Stanford University, USA
Scott Sternson – Janelia Research Campus, HHMI, USA
Ryohei Yasuda – Max Planck Florida Institute , USA