Protective effects of green tea polyphenols in the 6-OHDA rat model of Parkinson’s disease through inhibition of ROS-NO pathway.
Biological Psychiatry. 2007-12-01; 62(12): 1353-1362
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1. Biol Psychiatry. 2007 Dec 15;62(12):1353-62. Epub 2007 Jul 12.
Protective effects of green tea polyphenols in the 6-OHDA rat model of
Parkinson’s disease through inhibition of ROS-NO pathway.
Guo S(1), Yan J, Yang T, Yang X, Bezard E, Zhao B.
(1)State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics,
Academia Sinica, Beijing, China.
BACKGROUND: Nitric oxide (NO) and related pathways are thought to play an
important role in the pathogenesis of Parkinson’s disease (PD). Our in vitro
experiments suggested that green tea polyphenols (GTP) might protect dopamine
neurons through inhibition of NO and reactive oxygen species (ROS).
METHODS: Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated
dUTP Nick End Labeling assay, electron spin resonance spin trapping, enzyme
linked immunosorbent assay, and molecular biological methods were used to
investigate the effects of GTP in an unilateral 6-hydroxydopamine
(6-OHDA)-treated rat model of PD.
RESULTS: GTP treatment dose-dependently protected dopaminergic neurons by
preventing from midbrain and striatal 6-OHDA-induced increase in 1) both ROS and
NO levels, 2) lipid peroxidation, 3) nitrite/nitrate content, 4) inducible nitric
oxide synthase, and 5) protein-bound 3-nitro-tyrosine. Moreover, GTP treatment
dose-dependently preserved the free radical scavenging capability of both the
midbrain and the striatum.
CONCLUSIONS: These results support the in vivo protection of GTP against 6-OHDA
and suggest that GTP treatment might represent a neuroprotective treatment of PD.
PMID: 17624318 [Indexed for MEDLINE]