Nanoscale exploration of the extracellular space in the live brain by combining single carbon nanotube tracking and super-resolution imaging analysis
Methods. 2019-03-09; :
DOI: 10.1016/j.ymeth.2019.03.005
Read on PubMed
Paviolo C(1), Soria FN(2), Ferreira JS(3), Lee A(1), Groc L(3), Bezard E(2), Cognet L(4).
Author information:
(1)Université de Bordeaux, Laboratoire Photonique Numérique et Nanosciences, UMR
5298, 33400 Talence, France; Institut d’Optique & CNRS, LP2N UMR 5298, 33400
Talence, France.
(2)Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293,
33076 Bordeaux, France; CNRS, IMN UMR 5293, 33076 Bordeaux, France.
(3)Université de Bordeaux, Interdisciplinary Institute for Neurosciences, UMR
5297, 33076 Bordeaux, France; CNRS, IINS UMR 5297, 33076 Bordeaux, France.
(4)Université de Bordeaux, Laboratoire Photonique Numérique et Nanosciences, UMR
5298, 33400 Talence, France; Institut d’Optique & CNRS, LP2N UMR 5298, 33400
Talence, France. Electronic address: .
The brain extracellular space (ECS) is a system of narrow compartments whose
intricate nanometric structure has remained elusive until very recently.
Understanding such a complex organisation represents a technological challenge
that requires a technique able to resolve these nanoscopic spaces and
simultaneously characterize their rheological properties. We recently used
single-walled carbon nanotubes (SWCNTs) as near-infrared fluorescent probes to
map with nanoscale precision the local organization and rheology of the ECS. Here
we expand our method by tracking single nanotubes through super-resolution
imaging in rat organotypic hippocampal slices and acute brain slices from adult
mice, pioneering the exploration of the adult brain ECS at the nanoscale. We
found a highly heterogeneous ECS, where local rheological properties can change
drastically within few nanometres. Our results suggest differences in local ECS
diffusion environments in organotypic slices when compared to adult mouse slices.
Data obtained from super-resolved maps of the SWCNT trajectories indicate that
ECS widths may vary between brain tissue models, with a looser, less crowded
nano-environment in organotypic cultured slices.
Copyright © 2019. Published by Elsevier Inc.
DOI: 10.1016/j.ymeth.2019.03.005
PMID: 30862507