Kanamycin A-derived cationic lipids as vectors for gene transfection

Matthieu Sainlos, Michelle Hauchecorne, Noufissa Oudrhiri, Samia Zertal-Zidani, Abderrahim Aissaoui, Jean-Pierre Vigneron, Jean-Marie Lehn, Pierre Lehn
ChemBioChem. 2005-05-10; 6(6): 1023-1033
DOI: 10.1002/cbic.200400344

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1. Chembiochem. 2005 Jun;6(6):1023-33.

Kanamycin A-derived cationic lipids as vectors for gene transfection.

Sainlos M(1), Hauchecorne M, Oudrhiri N, Zertal-Zidani S, Aissaoui A, Vigneron
JP, Lehn JM, Lehn P.

Author information:
(1)Laboratoire de Chimie des Interactions Moléculaires, Collège de France, CNRS
UPR 285, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.

Cationic lipids nowadays constitute a promising alternative to recombinant
viruses for gene transfer. We have recently explored the transfection potential
of a new class of lipids based upon the use of aminoglycosides as cationic polar
headgroups. The encouraging results obtained with a first cholesterol derivative
of kanamycin A prompted us to investigate this family of vectors further, by
modulating the constituent structural units of the cationic lipid. For this
study, we have investigated the transfection properties of a series of new
derivatives based on a kanamycin A scaffold. The results primarily confirm that
aminoglycoside-based lipids are efficient vectors for gene transfection both in
vitro and in vivo (mouse airways). Furthermore, a combination of transfection and
physicochemical data revealed that some modifications of the constitutive
subunits of kanamycin A-based vectors were associated with substantial changes in
their transfection properties.

DOI: 10.1002/cbic.200400344
PMID: 15883979 [Indexed for MEDLINE]

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