Expression of Rho GTPases Rho-A and Rac1 in the adult and developing gerbil cerebellum.
International Journal of Developmental Neuroscience. 2008-11-01; 26(7): 723-732
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1. Int J Dev Neurosci. 2008 Nov;26(7):723-32. doi: 10.1016/j.ijdevneu.2008.07.010.
Epub 2008 Jul 17.
Expression of Rho GTPases Rho-A and Rac1 in the adult and developing gerbil
Richard V(1), Dulon D, Hafidi A.
(1)INSERM U 929, Neurobiologie de la douleur trigeminale, Faculté de Chirurgie
Dentaire, 11 boulevard Charles de Gaulle, 63000 Clermont-Ferrand, France.
Rho GTPases proteins are essential for cytoskeletal reorganization and play
important roles in the development of neuronal dendrites and axons. Several
studies have implicated two members of the Rho GTPase family Rho-A and Rac1
activities in the neuronal polarization and the formation of axons and dendrites.
In order to correlate cellular expressions of Rho-A and Rac1 with neuronal
polarity (axons versus dendrite formation) in the central nervous system, the
cerebellum and immunochemical techniques have been chosen. In the adult
cerebellar cortex differential pattern of distribution between Rho-A and Rac1 was
observed. While Rac1 expression was restricted to Purkinje cell (somata,
dendrites and axons), Rho-A was ubiquitously distributed within the cerebellar
cortex. Rac1 was localized in the Purkinje cell dendritic arborization (largest
and tiny dendrites) and in their axons. This pattern of distribution was also
observed during the postnatal development and followed the dendritic
morphogenesis of Purkinje cell. Rho-A was highly expressed in the adult Purkinje
cells somata, in cells of the granular layer, in glia within the white matter and
in axons. Intense staining was observed in Bergmann glia cell bodies and
processes. In the developing cerebellum, Rho-A was highly present in cells of the
external and internal granule layers and in the Purkinje cell layer. Bergmann
glia cell bodies and processes had the most intense staining during the
development. The present study reveals a high expression of Rac1 and Rho-A during
Purkinje cell neurites outgrowth period which occurred after birth in the
cerebellum. In addition Rho-A is highly expressed in granule cell progenitor
cells present in the external granular layer and therefore may play an important
role in granule cell progenitor migration.
PMID: 18682288 [Indexed for MEDLINE]