Effect of polyunsaturated fatty acids on endocannabinoid and N-acyl-ethanolamine levels in mouse adipocytes
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2008-01-01; 1781(1-2): 52-60
DOI: 10.1016/j.bbalip.2007.11.001
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1. Biochim Biophys Acta. 2008 Jan-Feb;1781(1-2):52-60. doi:
10.1016/j.bbalip.2007.11.001. Epub 2007 Nov 17.
Effect of polyunsaturated fatty acids on endocannabinoid and N-acyl-ethanolamine
levels in mouse adipocytes.
Matias I(1), Carta G, Murru E, Petrosino S, Banni S, Di Marzo V.
Author information:
(1)Endocannabinoid Research Group, Institute of Biomolecular Chemistry,
Consiglio Nazionale delle Ricerche, via dei Campi Flegrei 34, 80078 Pozzuoli
(Napoli), Italy.
The tissue concentrations of the endocannabinoids, 2-arachidonoylglycerol (2-AG)
and N-arachidonoyl-ethanolamine (anandamide), are altered in the adipose tissue
of mice fed a high fat diet. We have investigated here the effect on
endocannabinoid levels of incubation of mouse 3T3-F442A adipocytes with several
free polyunstaurated fatty acids (PUFAs), including linolenic acid (LA),
alpha-linolenic acid (ALA), arachidonic acid (AA) and docosahexaenoic acid
(DHA), as well as oleic acid (OA) and palmitic acid (PA). By using mass
spectrometric methods, we quantified the levels of endocannabinoids, of two
anandamide congeners, N-palmitoyl-ethanolamine (PEA) and N-oleoyl-ethanolamine
(OEA), and of fatty acids esterified in triacylglycerols or phospholipids, which
act as 2-AG and/or N-acyl-ethanolamine precursors. Incubation with AA strongly
elevated 2-AG levels and the amounts of AA esterified in triacylglycerols and on
glycerol carbon atom 2 (sn-2), but not 1 (sn-1), in phospholipids. Incubation
with DHA decreased 2-AG and anandamide levels and the amounts of AA esterified
on both the sn-2 and sn-1 position of phospholipids, but not on
triacylglycerols. PEA levels augmented following incubation of adipocytes with
OA and PA, with no corresponding changes in phospholipids and triacylglycerols.
We suggest that dietary PUFAs might modulate the levels of adipocyte
phospholipids that act as endocannabinoid precursors.
DOI: 10.1016/j.bbalip.2007.11.001
PMID: 18054340 [Indexed for MEDLINE]