Distinct Waves from the Hemogenic Endothelium Give Rise to Layered Lymphoid Tissue Inducer Cell Ontogeny
Cell Reports. 2020-08-01; 32(6): 108004
DOI: 10.1016/j.celrep.2020.108004
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1. Cell Rep. 2020 Aug 11;32(6):108004. doi: 10.1016/j.celrep.2020.108004.
Distinct Waves from the Hemogenic Endothelium Give Rise to Layered Lymphoid
Tissue Inducer Cell Ontogeny.
Simic M(1), Manosalva I(1), Spinelli L(1), Gentek R(1), Shayan RR(1), Siret
C(1), Girard-Madoux M(1), Wang S(1), de Fabritus L(1), Verschoor J(1), Kerdiles
YM(1), Bajenoff M(1), Stumm R(2), Golub R(3), van de Pavert SA(4).
Author information:
(1)Aix-Marseille Université, Centre National de la Recherche Scientifique
(CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM),
Centre d’Immunologie de Marseille-Luminy (CIML), 13288 Marseille, France.
(2)Institute of Pharmacology and Toxicology, Jena University Hospital, Friedrich
Schiller University Jena, 07747 Jena, Germany.
(3)Institut Pasteur, Immunology Department, Lymphopoiesis Unit, INSERM U668,
University Paris Diderot, Paris, France.
(4)Aix-Marseille Université, Centre National de la Recherche Scientifique
(CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM),
Centre d’Immunologie de Marseille-Luminy (CIML), 13288 Marseille, France.
Electronic address: .
During embryogenesis, lymphoid tissue inducer (LTi) cells are essential for
lymph node organogenesis. These cells are part of the innate lymphoid cell (ILC)
family. Although their earliest embryonic hematopoietic origin is unclear, other
innate immune cells have been shown to be derived from early hemogenic
endothelium in the yolk sac as well as the aorta-gonad-mesonephros. A proper
model to discriminate between these locations was unavailable. In this study,
using a Cxcr4-CreERT2 lineage tracing model, we identify a major contribution
from embryonic hemogenic endothelium, but not the yolk sac, toward LTi
progenitors. Conversely, embryonic LTi cells are replaced by hematopoietic stem
cell-derived cells in adults. We further show that, in the fetal liver, common
lymphoid progenitors differentiate into highly dynamic alpha-lymphoid precursor
cells that, at this embryonic stage, preferentially mature into LTi precursors
and establish their functional LTi cell identity only after reaching the
periphery.
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
DOI: 10.1016/j.celrep.2020.108004
PMID: 32783932 [Indexed for MEDLINE]
Conflict of interest statement: Declaration of Interests The authors declare no
competing interests.