ATP P2X receptors downregulate AMPA receptor trafficking and postsynaptic efficacy in hippocampal neurons.
Neuron. 2014-07-01; 83(2): 417-430
DOI: 10.1016/j.neuron.2014.06.005
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1. Neuron. 2014 Jul 16;83(2):417-430. doi: 10.1016/j.neuron.2014.06.005.
ATP P2X receptors downregulate AMPA receptor trafficking and postsynaptic
efficacy in hippocampal neurons.
Pougnet JT(1), Toulme E(1), Martinez A(1), Choquet D(2), Hosy E(2), Boué-Grabot
E(3).
Author information:
(1)Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293,
33000 Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293,
33000 Bordeaux, France.
(2)Université de Bordeaux, Institut Interdisciplinaire des Neurosciences, UMR
5297, 33000 Bordeaux, France; CNRS, Institut Interdisciplinaire des
Neurosciences, UMR 5297, 33000 Bordeaux, France.
(3)Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293,
33000 Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293,
33000 Bordeaux, France. Electronic address: .
Comment in
Neuron. 2014 Jul 16;83(2):257-9.
P2X receptors (P2XRs) are ATP-gated cation channels widely expressed in the brain
where they mediate action of extracellular ATP released by neurons or glia.
Although purinergic signaling has multiple effects on synaptic transmission and
plasticity, P2XR function at brain synapses remains to be established. Here, we
show that activation of postsynaptic P2XRs by exogenous ATP or
noradrenaline-dependent glial release of endogenous ATP decreases the amplitude
of miniature excitatory postsynaptic currents and AMPA-evoked currents in
cultured hippocampal neurons. We also observed a P2X-mediated depression of field
potentials recorded in CA1 region from brain slices. P2X2Rs trigger
dynamin-dependent internalization of AMPA receptors (AMPARs), leading to reduced
surface AMPARs in dendrites and at synapses. AMPAR alteration required calcium
influx through opened ATP-gated channels and phosphatase or CamKII activities.
These findings indicate that postsynaptic P2XRs play a critical role in
regulating the surface expression of AMPARs and thereby regulate the synaptic
strength.
Copyright © 2014 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.neuron.2014.06.005
PMID: 25033184 [Indexed for MEDLINE]