Age-Dependent Modulation of Layer V Pyramidal Neuron Excitability in the Mouse Primary Motor Cortex by D1 Receptor Agonists and Antagonists
Neuroscience. 2024-01-01; 536: 21-35
DOI: 10.1016/j.neuroscience.2023.11.006
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1. Neuroscience. 2023 Nov 11;536:21-35. doi: 10.1016/j.neuroscience.2023.11.006.
Online ahead of print.
Age-Dependent Modulation of Layer V Pyramidal Neuron Excitability in the Mouse
Primary Motor Cortex by D1 Receptor Agonists and Antagonists.
Plateau V(1), Baufreton J(1), Le Bon-Jégo M(2).
Author information:
(1)Université de Bordeaux, Institut des Maladies Neurodégénératives, 33076
Bordeaux, France; CNRS UMR 5293, Institut des Maladies Neurodégénératives, 33076
Bordeaux, France.
(2)Université de Bordeaux, Institut des Maladies Neurodégénératives, 33076
Bordeaux, France; CNRS UMR 5293, Institut des Maladies Neurodégénératives, 33076
Bordeaux, France. Electronic address: .
The primary motor cortex (M1) receives dopaminergic (DAergic) projections from
the midbrain which play a key role in modulating motor and cognitive processes,
such as motor skill learning. However, little is known at the level of
individual neurons about how dopamine (DA) and its receptors modulate the
intrinsic properties of the different neuronal subpopulations in M1 and if this
modulation depends on age. Using immunohistochemistry, we first mapped the cells
expressing the DA D1 receptor across the different layers in M1, and quantified
the number of pyramidal neurons (PNs) expressing the D1 receptor in the
different layers, in young and adult mice. This work reveals that the spatial
distribution and the molecular profile of D1 receptor-expressing neurons (D1+)
across M1 layers do not change with age. Then, combining whole-cell patch-clamp
recordings and pharmacology, we explored ex vivo in young and adult mice the
impact of activation or blockade of D1 receptors on D1+ PN intrinsic properties.
While the bath application of the D1 receptor agonist induced an increase in the
excitability of layer V PNs both in young and adult, we identified a distinct
modulation of intrinsic electrical properties of layer V D1+ PNs by D1 receptor
antagonist depending on the age of the animal.
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
DOI: 10.1016/j.neuroscience.2023.11.006
PMID: 37952579