A new cerebral hemorrhage model in cynomolgus macaques created by injection of autologous anticoagulated blood into the brain.
Journal of Clinical Neuroscience. 2011-07-01; 18(7): 955-960
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1. J Clin Neurosci. 2011 Jul;18(7):955-60. doi: 10.1016/j.jocn.2010.11.038. Epub
2011 May 20.
A new cerebral hemorrhage model in cynomolgus macaques created by injection of
autologous anticoagulated blood into the brain.
Zhu H(1), Li Q, Feng M, Chen YX, Li H, Sun JJ, Zhao CH, Wang RZ, Bezard E, Qin C.
(1)Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences
and Peking Union Medical College, No. 5 Panjiayuan, Nanli, Chaoyang District,
Beijing 100021, China.
The aim of this study was to establish and validate a clinically relevant model
of intracerebral hemorrhage (ICH) via injection of autologous blood into the
brains of cynomolgus macaques (Macaca fascicularis). Eight male cynomolgus
macaques received 1.5 mL of fresh anticoagulated autologous femoral artery blood
into the inner side of the claustrum near the right basal ganglia under
stereotactic guidance. Animals were evaluated with MRI and positron emission
tomography (PET) scanning before and 24 hours after surgery and once per week
thereafter. A neurological deficit scale was used to assess the animals on days
1, 2, 3, 7, 14, 21, and 28 after surgery. Animals showed focal neurological signs
corresponding to the MRI-located hematoma. The behavioral impairment
progressively ameliorated over time, but never fully resolved. The hematoma was
absorbed over time but was still present 4 weeks after surgery, with persistent
metabolic deficit detected using PET scanning. Histological examinations
confirmed the in vivo findings. This ICH model in a non-human primate mimics
human ICH in the basal ganglia and may be useful for assessing the safety and
efficacy of neuroprotective agents.
Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.
PMID: 21601461 [Indexed for MEDLINE]