Tools for investigating peptide-protein interactions: Peptide incorporation of environment-sensitive fluorophores via on-resin derivatization
Nat Protoc. 2007-12-01; 2(12): 3201-3209
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1. Nat Protoc. 2007;2(12):3201-9.
Tools for investigating peptide-protein interactions: peptide incorporation of
environment-sensitive fluorophores via on-resin derivatization.
Sainlos M(1), Imperiali B.
(1)Department of Chemistry, Massachusetts Institute of Technology, 77
Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.
This protocol presents the peptide incorporation of environment-sensitive
fluorophores derived from the dimethylaminophthalimide family. The procedure
utilizes anhydride precursors of 4-dimethylaminophthalimide (4-DMAP) or
6-dimethylaminonaphthalimide (6-DMN), whose syntheses are described in a related
protocol from these authors. In this protocol, the fluorophores are directly
incorporated after solid-phase peptide synthesis (SPPS) via on-resin
derivatization of peptides prepared using commercially available diamino acids,
which are Alloc-protected on the side-chain amino group. The time required to
complete the procedure depends on the size and number of peptides targeted. As an
alternative to this approach, the corresponding fluorescent amino acids can be
obtained in an Fmoc-protected form for convenient use as building blocks in SPPS.
This option is described in a related protocol by these authors.
PMID: 18079720 [Indexed for MEDLINE]