TNF-alpha influences the lateral dynamics of TNF receptor I in living cells

Meike Heidbreder, Christin Zander, Sebastian Malkusch, Darius Widera, Barbara Kaltschmidt, Christian Kaltschmidt, Deepak Nair, Daniel Choquet, Jean-Baptiste Sibarita, Mike Heilemann
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2012-10-01; 1823(10): 1984-1989
DOI: 10.1016/j.bbamcr.2012.06.026

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1. Biochim Biophys Acta. 2012 Oct;1823(10):1984-9. doi:
10.1016/j.bbamcr.2012.06.026. Epub 2012 Jun 27.

TNF-α influences the lateral dynamics of TNF receptor I in living cells.

Heidbreder M(1), Zander C, Malkusch S, Widera D, Kaltschmidt B, Kaltschmidt C,
Nair D, Choquet D, Sibarita JB, Heilemann M.

Author information:
(1)Julius-Maximilians-Universität, Department of Biotechnology & Biophysics,
Würzburg, Germany.

In mammalian cells, inflammation is mainly mediated by the binding of tumor
necrosis factor alpha to tumor necrosis factor receptor 1. In this study, we
investigated lateral dynamics of TNF-R1 before and after ligand binding using
high-density single-particle tracking in combination with photoactivated
localization microscopy. Our single-molecule data indicates the presence of tumor
necrosis factor receptor 1 with different mobilities in the plasma membrane,
suggesting different molecular organizations. Cholesterol depletion led to a
decrease of slow receptor species and a strong increase in the average diffusion
coefficient. Moreover, as a consequence of tumor necrosis factor-alpha treatment,
the mean diffusion coefficient moderately increased while its distribution
narrowed. Based on our observation, we propose a refined mechanism on the
structural arrangement and activation of tumor necrosis factor receptor 1 in the
plasma membrane.

Copyright © 2012 Elsevier B.V. All rights reserved.

DOI: 10.1016/j.bbamcr.2012.06.026
PMID: 22749881 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus