Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes
Biochemical and Biophysical Research Communications. 2012-01-01; 417(1): 409-413
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1. Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13. doi:
10.1016/j.bbrc.2011.11.129. Epub 2011 Dec 7.
Single Particle Tracking reveals two distinct environments for CD4 receptors at
the surface of living T lymphocytes.
Mascalchi P(1), Lamort AS, Salomé L, Dumas F.
(1)CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse,
We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T
lymphocytes at 20°C and 37°C by Single Particle Tracking using Quantum Dots. We
found that the receptors presented two major distinct behaviors that were not
equally affected by temperature changes. About half of the receptors showed a
random diffusion with a diffusion coefficient increasing upon raising the
temperature. The other half of the receptors was permanently or transiently
confined with unchanged dynamics on raising the temperature. These observations
suggest that two distinct subpopulations of CD4 receptors with different
environments are present at the surface of living T lymphocytes.
Copyright © 2011 Elsevier Inc. All rights reserved.
PMID: 22166195 [Indexed for MEDLINE]