Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes

Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13. doi: 10.1016/j.bbrc.2011.11.129. Epub 2011 Dec 7.

Abstract

We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T lymphocytes at 20°C and 37°C by Single Particle Tracking using Quantum Dots. We found that the receptors presented two major distinct behaviors that were not equally affected by temperature changes. About half of the receptors showed a random diffusion with a diffusion coefficient increasing upon raising the temperature. The other half of the receptors was permanently or transiently confined with unchanged dynamics on raising the temperature. These observations suggest that two distinct subpopulations of CD4 receptors with different environments are present at the surface of living T lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4 Antigens / analysis*
  • Cell Membrane / chemistry*
  • Cell Membrane / immunology
  • HIV / immunology*
  • Humans
  • Jurkat Cells
  • T-Lymphocytes / chemistry*
  • T-Lymphocytes / immunology

Substances

  • CD4 Antigens