Principles of Total Internal Reflection Microscopy (TIRFM).
Cold Spring Harb Protoc. 2007-10-01; 2007(10): pdb.top24
DOI: 10.1101/pdb.top24
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1. CSH Protoc. 2007 Oct 1;2007:pdb.top24. doi: 10.1101/pdb.top24.
Principles of Total Internal Reflection Microscopy (TIRFM).
Zenisek D, Perrais D.
INTRODUCTIONTotal internal reflection fluorescence microscopy (TIRFM) is a
powerful technique for studying events that occur near a cell surface. The
technique allows selective imaging of fluorescent molecules that are closest to a
high refractive index substance such as glass. TIRFM has been used to study (1)
exocytosis of single synaptic vesicles stained with FM1-43 in living goldfish
retinal bipolar neurons and (2) exocytosis of single dense core granules stained
with neuropeptide Y-enhanced green fluorescent protein (NPY-EGFP) in living
bovine chromaffin cells. This article describes the basic theory behind TIRFM and
provides an overview for setting up a TIRFM system.
PMID: 21356959