Is otospiralin inner ear specific? Evidence for its expression in mouse brain.
International Journal of Developmental Neuroscience. 2009-02-01; 27(1): 87-96
DOI: 10.1016/j.ijdevneu.2008.09.001
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1. Int J Dev Neurosci. 2009 Feb;27(1):87-96. doi: 10.1016/j.ijdevneu.2008.09.001.
Epub 2008 Sep 11.
Is otospiralin inner ear specific? Evidence for its expression in mouse brain.
Decourt B(1), Hillman D, Bouleau Y, Dulon D, Hafidi A.
Author information:
(1)INSERM U587 and University of Bordeaux 2, Institut des Neurosciences de
Bordeaux, Bordeaux F-33076, France.
The small protein otospiralin has initially been identified as an inner ear
specific molecule. However, compelling evidence from high throughput sequencing
projects suggested that otospiralin is likely expressed in the central nervous
system. Here, we tested this hypothesis using a combination of molecular biology,
immunological, and histological techniques, and found that otospiralin is
expressed in numerous regions of the central nervous system in mouse. In situ
hybridization and immunohistochemistry revealed that otospiralin is widely
expressed in neuronal cell bodies and glia. Ultrastructural observations in the
cerebral cortex located the small protein in close proximity to membranous
organelles in perikarya, the inner face of post-synaptic neuronal membranes, and
in astrocytic processes. These results are in agreement with the predicted
structure of the protein which revealed a single N-terminal transmembrane helix
domain followed by a C-terminus cytosolic tail. Interestingly, 2 weeks after a
mechanical trauma in the cerebral cortex, otospiralin expression increased in
reactive astrocytes located within the vicinity of the site of injury, but not in
neurons. Collectively, our observations suggest that otospiralin is possibly
involved in signaling pathways, and could play a role in repair mechanisms
subsequent to an injury in the central nervous system.
DOI: 10.1016/j.ijdevneu.2008.09.001
PMID: 18832023 [Indexed for MEDLINE]