Intracranial graft of bioresorbable polymer scaffolds loaded with human Dental Pulp Stem Cells in stab wound murine injury model
Methods in Cell Biology. 2024-01-01; : 237-254
DOI: 10.1016/bs.mcb.2024.03.011
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Manero-Roig I(1), Polo Y(2), Pardo-Rodríguez B(3), Luzuriaga J(4),
Basanta-Torres R(3), Martín-Aragón D(3), Romayor I(5), Martín-Colomo S(6),
Márquez J(3), Gomez-Santos L(3), Lanore F(7), Humeau Y(7), Ibarretxe G(8),
Eguizabal C(9), Larrañaga A(10), Pineda JR(11).
Author information:
(1)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain; University of
Bordeaux, CNRS, IINS, UMR 5297, Bordeaux, France.
(2)Polimerbio SL, Donostia-San Sebastián, Spain.
(3)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain.
(4)Department of Physiology, Faculty of Medicine and Nursing, University of the
Basque Country (UPV/EHU), Leioa, Spain.
(5)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain; Cell Therapy, Stem
Cells and Tissues Group, Biobizkaia Health Research Institute, Barakaldo, Spain;
Advanced Therapies Unit, Basque Center for Blood Transfusion and Human Tissues,
Bizkaia, Spain.
(6)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain; Group of Science and
Engineering of Polymeric Biomaterials (ZIBIO Group), Department of Mining,
Metallurgy Engineering and Materials Science, POLYMAT, University of the Basque
Country (UPV/EHU), Bilbao, Spain.
(7)University of Bordeaux, CNRS, IINS, UMR 5297, Bordeaux, France.
(8)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain. Electronic address:
.
(9)Cell Therapy, Stem Cells and Tissues Group, Biobizkaia Health Research
Institute, Barakaldo, Spain; Advanced Therapies Unit, Basque Center for Blood
Transfusion and Human Tissues, Bizkaia, Spain. Electronic address:
.
(10)Group of Science and Engineering of Polymeric Biomaterials (ZIBIO Group),
Department of Mining, Metallurgy Engineering and Materials Science, POLYMAT,
University of the Basque Country (UPV/EHU), Bilbao, Spain. Electronic address:
.
(11)Department of Cell Biology and Histology, Faculty of Medicine and Nursing,
University of the Basque Country (UPV/EHU), Leioa, Spain; Achucarro Basque
Center for Neuroscience Fundazioa, Leioa, Spain. Electronic address:
.
The prevalence of central nervous system (CNS) dysfunction as a result of
disease or trauma remains a clinically unsolved problem which is raising
increased awareness in our aging society. Human Dental Pulp Stem Cells (hDPSCs)
are excellent candidates to be used in tissue engineering and regenerative
therapies of the CNS due to their neural differentiation ability and lack of
tumorigenicity. Accordingly, they have been successfully used in animal models
of spinal cord injury, stroke and peripheral neuropathies. The ideal therapy in
brain injury should combine strategies aiming to protect the damaged lesion and,
at the same time, accelerate brain tissue regeneration, thus promoting fast
recovery while minimizing side or long-term effects. The use of bioresorbable
nanopatterned poly(lactide-co-ɛ-caprolactone) (PLCL) polymeric scaffolds as
hDPCSs carriers can represent an advantage for tissue regeneration. In this
chapter, we describe the surgical procedures to implant functionalized
bioresorbable scaffolds loaded with hDPSCs to improve the brain lesion
microenvironment in an intracranial stab wound injury model severing the rostral
migratory stream (RMS) that connects the brain subventricular zone (SVZ) and the
olfactory bulb in nude mice. Additionally, we also describe the technical steps
after animal sacrifice for histological tissue observation and characterization.
Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text
and data mining, AI training, and similar technologies.
DOI: 10.1016/bs.mcb.2024.03.011
PMID: 38880526 [Indexed for MEDLINE]
Conflict of interest statement: Disclosures I.M.R., Y.P., B.P.R., J.L., R.B.T.,
D.M.A., I.R., S.M.C., J.M., L.G.S., G.I., F.L., Y.H., C.E., A.L., J.R.P. have no
conflict of interest to disclose.