Imaging of molecular surface dynamics in brain slices using single-particle tracking
Nat Commun. 2014-01-16; 5(1):
DOI: 10.1038/ncomms4024
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1. Nat Commun. 2014;5:3024. doi: 10.1038/ncomms4024.
Imaging of molecular surface dynamics in brain slices using single-particle
tracking.
Biermann B(1), Sokoll S(2), Klueva J(3), Missler M(4), Wiegert JS(5), Sibarita
JB(6), Heine M(3).
Author information:
(1)1] Research Group Molecular Physiology, Leibniz-Institute of Neurobiology,
Brenneckestrasse 6, D-39118 Magdeburg, Germany [2] Institute of Anatomy and
Molecular Neurobiology, Westfälische Wilhelms-University, Vesaliusweg 2-4,
D-48149 Münster, Germany [3].
(2)1] Research Group Molecular Physiology, Leibniz-Institute of Neurobiology,
Brenneckestrasse 6, D-39118 Magdeburg, Germany [2].
(3)Research Group Molecular Physiology, Leibniz-Institute of Neurobiology,
Brenneckestrasse 6, D-39118 Magdeburg, Germany.
(4)Institute of Anatomy and Molecular Neurobiology, Westfälische
Wilhelms-University, Vesaliusweg 2-4, D-48149 Münster, Germany.
(5)Center for Molecular Neurobiology (ZMNH), University Medical Center
Hamburg-Eppendorf, Falkenried 94, D-20251 Hamburg, Germany.
(6)1] University of Bordeaux, Interdisciplinary Institute for Neuroscience,
F-33000 Bordeaux, France [2] CNRS UMR 5297, F-33000 Bordeaux, France.
Organization of signalling molecules in biological membranes is crucial for
cellular communication. Many receptors, ion channels and cell adhesion molecules
are associated with proteins important for their trafficking, surface
localization or function. These complexes are embedded in a lipid environment of
varying composition. Binding affinities and stoichiometry of such complexes were
so far experimentally accessible only in isolated systems or monolayers of cell
culture. Visualization of molecular dynamics within signalling complexes and
their correlation to specialized membrane compartments demand high temporal and
spatial resolution and has been difficult to demonstrate in complex tissue like
brain slices. Here we demonstrate the feasibility of single-particle tracking
(SPT) in organotypic brain slices to measure molecular dynamics of lipids and
transmembrane proteins in correlation to synaptic membrane compartments. This
method will provide important information about the dynamics and organization of
surface molecules in the complex environment of neuronal networks within brain
slices.
DOI: 10.1038/ncomms4024
PMCID: PMC3905702
PMID: 24429796 [Indexed for MEDLINE]