Human GluR6c, a functional splicing variants of GluR6, is mainly expressed in non-nervous cells.
Neuroscience Letters. 2008-03-01; 434(1): 77-82
DOI: 10.1016/j.neulet.2008.01.049
Lire sur PubMed
1. Neurosci Lett. 2008 Mar 21;434(1):77-82. doi: 10.1016/j.neulet.2008.01.049. Epub
2008 Feb 2.
Human GluR6c, a functional splicing variants of GluR6, is mainly expressed in
non-nervous cells.
Barbon A(1), Gervasoni A, LaVia L, Orlandi C, Jaskolski F, Perrais D, Barlati S.
Author information:
(1)Division of Biology and Genetics, Department of Biomedical Sciences and
Biotechnologies, University of Brescia, Viale Europa 11, 25123 Brescia, Italy.
Kainate-type glutamate receptors (KARs) are receptor channels with a variety of
distinct physiological functions in synaptic transmission, depending on their
sub-cellular location in functional neuronal compartments. The kainate receptor
subunit GluR6 presents different splice variants involving the C-terminal domain,
namely GluR6a, GluR6b and GluR6c. In this study, we report the analysis of the
three human splicing isoforms and in particular of the uncharacterized hGluR6c.
When expressed in COS-7 cells, hGluR6a receptor subunit was highly present on the
surface of the plasma membrane, whereas hGluR6b and hGluRc were poorly
transported to the membrane. Electrophysiological studies of homomeric receptors
showed that hGluR6c subunit can generate functional receptors with
characteristics similar to the GluR6b variant. mRNA expression analysis
demonstrated that hGluR6c variant is mainly expressed in non-neuronal cells and
barely expressed in neuronal ones. Interestingly, undifferentiated NT2 cells
expressing only the hGluR6c isoform, during neuronal differentiation induced by
retinoic acid, increased the expression level of the neuronal form hGluR6a with a
parallel decreased of hGluR6c. Overall, our data indicate that hGluR6c might have
unique properties in non-nervous cells and in the first stages of CNS
development.
DOI: 10.1016/j.neulet.2008.01.049
PMID: 18289788 [Indexed for MEDLINE]