Dietary N-3 PUFA deficiency affects sleep-wake activity in basal condition and in response to an inflammatory challenge in mice
Brain, Behavior, and Immunity. 2019-05-01; :
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Decoeur F(1), Benmamar-Badel A(1), Leyrolle Q(1), Persillet M(1), Layé S(1), Nadjar A(2).
(1)Univ. Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000 Bordeaux,France.
(2)Univ. Bordeaux, INRA, Bordeaux INP, NutriNeuro, UMR 1286, F-33000 Bordeaux, France. Electronic address: .
Essential polyunsaturated fatty acids (PUFA) from the n-3 and n-6 series
constitute the building blocks of brain cell membranes where they regulate most
aspects of cell physiology. They are either biosynthesized from their dietary
precursors or can be directly sourced from the diet. An overall increase in the
dietary n-6/n-3 PUFA ratio, as observed in the Western diet, leads to reduced n-3
PUFAs in tissues that include the brain. Some clinical studies have shown a
positive correlation between dietary n-3 PUFA intake and sleep quantity, yet
evidence is still sparse. We here used a preclinical model of dietary n-3 PUFA
deficiency to assess the precise relationship between dietary PUFA intake and
sleep/wake activity. Using electroencephalography (EEG)/electromyography (EMG)
recordings on n-3 PUFA deficient or sufficient mice, we showed that dietary PUFA
deficiency affects the architecture of sleep-wake activity and the oscillatory
activity of cortical neurons during sleep. In a second part of the study, and
since PUFAs are a potent modulator of inflammation, we assessed the effect of
dietary n-3 PUFA deficiency on the sleep response to an inflammatory stimulus
known to modulate sleep/wake activity. We injected mice with the endotoxin
lipopolysaccharide (LPS) and quantified the sleep response across the following
12 h. Our results revealed that n-3 PUFA deficiency affects the sleep response in
basal condition and after a peripheral immune challenge. More studies are now
required aimed at deciphering the molecular mechanisms underlying the intimate
relationship between n-3 PUFAs and sleep/wake activity.
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