A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis
MBoC. 2015-07-01; 26(13): 2519-2534
DOI: 10.1091/mbc.E14-11-1527
Lire sur PubMed
1. Mol Biol Cell. 2015 Jul 1;26(13):2519-34. doi: 10.1091/mbc.E14-11-1527. Epub 2015
May 6.
A quantitative imaging-based screen reveals the exocyst as a network hub
connecting endocytosis and exocytosis.
Jose M(1), Tollis S(2), Nair D(3), Mitteau R(2), Velours C(2), Massoni-Laporte
A(2), Royou A(4), Sibarita JB(5), McCusker D(6).
Author information:
(1)Dynamics of Cell Growth and Division, European Institute of Chemistry and
Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires,
CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France Centre for
Neuroscience, Indian Institute of Science, Bangalore 560012, India.
(2)Dynamics of Cell Growth and Division, European Institute of Chemistry and
Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires,
CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
(3)Centre for Neuroscience, Indian Institute of Science, Bangalore 560012, India
Institut Interdisciplinaire de Neurosciences, CNRS UMR 5297, Université Bordeaux,
F-33000 Bordeaux, France.
(4)Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université
Bordeaux, F-33000 Bordeaux, France Institut de Biochimie et Génétique
Cellulaires, CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France.
(5)Institut Interdisciplinaire de Neurosciences, CNRS UMR 5297, Université
Bordeaux, F-33000 Bordeaux, France.
(6)Dynamics of Cell Growth and Division, European Institute of Chemistry and
Biology, F-33607 Bordeaux, France Institut de Biochimie et Génétique Cellulaires,
CNRS UMR 5095, Université Bordeaux, F-33000 Bordeaux, France
.
The coupling of endocytosis and exocytosis underlies fundamental biological
processes ranging from fertilization to neuronal activity and cellular polarity.
However, the mechanisms governing the spatial organization of endocytosis and
exocytosis require clarification. Using a quantitative imaging-based screen in
budding yeast, we identified 89 mutants displaying defects in the localization of
either one or both pathways. High-resolution single-vesicle tracking revealed
that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle
dynamics in opposite ways. The endocytic and exocytic pathways display strong
interdependence during polarity establishment while being more independent during
polarity maintenance. Systems analysis identified the exocyst complex as a key
network hub, rich in genetic interactions with endocytic and exocytic components.
Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and
interspersed endocytic and exocytic domains compared with control cells. These
data are consistent with an important role for the exocyst in coordinating
endocytosis and exocytosis.
© 2015 Jose et al. This article is distributed by The American Society for Cell
Biology under license from the author(s). Two months after publication it is
available to the public under an Attribution–Noncommercial–Share Alike 3.0
Unported Creative Commons License
(http://creativecommons.org/licenses/by-nc-sa/3.0).
DOI: 10.1091/mbc.E14-11-1527
PMCID: PMC4571305
PMID: 25947137 [Indexed for MEDLINE]