Isolation of Hypothalamic Microglia from Freshly Perfused Adult Mouse Brain by Magnetic-Activated Cell Sorting
JoVE. 2024-09-06; (211):
DOI: 10.3791/66769
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https://www.bordeaux-neurocampus.fr/12104
Kyriakidou E(#)(1), Cutugno G(#)(1), Allard C(2), Nadjar A(3).
Author information:
(1)University of Bordeaux, INSERM, Neurocentre Magendie.
(2)University of Bordeaux, INSERM, Neurocentre Magendie;
.
(3)University of Bordeaux, INSERM, Neurocentre Magendie;
.
(#)Contributed equally
Microglia, as the resident macrophages of the brain, are essential for
maintaining brain homeostasis. They shape neuronal circuits during development,
survey their environment for debris or dead cells, as well as respond to
infection and injury in the brain, among many other functions. However, their
important role in neurodevelopment and synaptic plasticity and pathophysiology
has not been fully defined, highlighting the need for further investigation. To
gain a more comprehensive understanding of the role of microglia in these
processes, we need to isolate microglia and characterize them genetically,
metabolically, and functionally. However, the isolation of microglia from adult
mice, especially from small brain structures, is challenging as they represent a
small percentage of the total brain cells, and the yield of isolated microglia
is often too low. Here, the magnetic isolation of microglia using CD11b+
microbeads allows us to sort microglial cells from the hypothalamus of a freshly
perfused adult mouse brain. The current method allows us to achieve relatively
high purity and yield in a short period while maintaining cell viability.
DOI: 10.3791/66769
PMID: 39311614 [Indexed for MEDLINE]