Jonathan Elegheert
Career
After studying medicine (BSc) and biochemistry (MSc), I obtained my PhD in 2012 from Ghent University (Belgium), where I studied the structural biology of Colony-Stimulating Factor 1 (CSF-1) cytokine signalling in the lab of Prof. Savvas N. Savvides as a Research Foundation – Flanders (FWO)-funded doctoral fellow. In 2012, I joined the Division of Structural Biology (STRUBI) at the University of Oxford (UK) as an EMBO- and Marie Curie Actions-funded postdoctoral fellow. There, I transitioned into molecular neuroscience and investigated the structural biology of synaptic protein complexes, in the lab of Prof. A. Radu Aricescu. In 2018, I joined the IINS as a team leader enabled by an Initiative of Excellence (IdEx) Junior Chair (University of Bordeaux) and a Neurocampus starting package (Conseil Régional de la Nouvelle Aquitaine). In 2019, I was recruited to the CNRS, was a laureate of the CNRS-Inserm ATIP-Avenir program and obtained an ERC Starting Grant.
Publications
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GRID1/GluD1 homozygous variants linked to intellectual disability and spastic paraplegia impair mGlu1/5 receptor signaling and excitatory synapses
Dévina C. Ung, Nicolas Pietrancosta, Elena Baz Badillo, Brigitt Raux, Daniel Tapken, Andjela Zlatanovic, Adrien Doridant, Ben Pode-Shakked, Annick Raas-Rothschild, Orly Elpeleg, Bassam Abu-Libdeh, Nasrin Hamed, Marie-Amélie Papon, Sylviane Marouillat, Rose-Anne Thépault, Giovanni Stevanin, Jonathan Elegheert, Mathieu Letellier, Michael Hollmann, Bertrand Lambolez, Ludovic Tricoire, Annick Toutain, Régine Hepp, Frédéric Laumonnier.
Mol Psychiatry. 2024-02-28.
10.1038/s41380-024-02469-w -
High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus.
Ester Behiels, Jonathan Elegheert.
Methods in Molecular Biology. 2021-01-01. : 83-104.
10.1007/978-1-0716-1406-8_4 -
A synthetic synaptic organizer protein restores glutamatergic neuronal circuits.
Suzuki K, Elegheert J, Song I, Sasakura H, Senkov O, Matsuda K, Kakegawa W, Clayton AJ, Chang VT, Ferrer-Ferrer M, Miura E, Kaushik R, Ikeno M, Morioka Y, Takeuchi Y, Shimada T, Otsuka S, Stoyanov S, Watanabe M, Takeuchi K, Dityatev A, Aricescu AR, Yuzaki M.
Science. . 369(6507) : 1052-1053.
10.1126/science.abb4853 -
A synthetic synaptic organizer protein restores glutamatergic neuronal circuits
Kunimichi Suzuki, Jonathan Elegheert, Inseon Song, Hiroyuki Sasakura, Oleg Senkov, Wataru Kakegawa, Amber J. Clayton, Veronica T. Chang, Maura Ferrer-Ferrer, Eriko Miura, Rahul Kaushik, Masashi Ikeno, Yuki Morioka, Yuka Takeuchi, Tatsuya Shimada, Shintaro Otsuka, Stoyan Stoyanov, Masahiko Watanabe, Kosei Takeuchi, Alexander Dityatev, A. Radu Aricescu, Michisuke Yuzaki.
. 2020-02-27.
10.1101/2020.02.27.967836 -
Pumping the brakes: suppression of synapse development by MDGA-neuroligin interactions.
Steven A Connor, Jonathan Elegheert, Yicheng Xie, Ann Marie Craig.
Current Opinion in Neurobiology. 2019-08-01. 57 : 71-80.
10.1016/j.conb.2019.01.002 -
Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins
Jonathan Elegheert, Ester Behiels, Benjamin Bishop, Suzanne Scott, Rachel E. Woolley, Samuel C. Griffiths, Eamon F. X. Byrne, Veronica T. Chang, David I. Stuart, E. Yvonne Jones, Christian Siebold, A. Radu Aricescu.
Nat Protoc. 2018-11-19. 13(12) : 2991-3017.
10.1038/s41596-018-0075-9 -
Calibration-free in vitro quantification of protein homo-oligomerization using commercial instrumentation and free, open source brightness analysis software
Rory Nolan, Luis A. Alvarez, Samuel C. Griffiths, Jonathan Elegheert, Christian Siebold, Sergi Padilla-Parra.
JoVE. 2018-07-17. (137)
10.3791/58157 -
Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands
Jonathan Elegheert, Ann Brigé, Jozef Van Beeumen, Savvas N. Savvides.
FEBS Lett. 2017-09-15. 591(20) : 3391-3401.
10.1002/1873-3468.12833 -
Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins.
Jonathan Elegheert, Vedrana Cvetkovska, Amber J. Clayton, Christina Heroven, Kristel M. Vennekens, Samuel N. Smukowski, Michael C. Regan, Wanyi Jia, Alexandra C. Smith, Hiro Furukawa, Jeffrey N. Savas, Joris de Wit, Jo Begbie, Ann Marie Craig, A. Radu Aricescu.
Neuron. 2017-09-01. 96(1) : 242-244.
10.1016/j.neuron.2017.07.040 -
Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins
Jonathan Elegheert, Vedrana Cvetkovska, Amber J. Clayton, Christina Heroven, Kristel M. Vennekens, Samuel N. Smukowski, Michael C. Regan, Wanyi Jia, Alexandra C. Smith, Hiro Furukawa, Jeffrey N. Savas, Joris de Wit, Jo Begbie, Ann Marie Craig, A. Radu Aricescu.
Neuron. 2017-08-01. 95(4) : 896-913.e10.
10.1016/j.neuron.2017.07.040 -
nandb-number and brightness in R with a novel automatic detrending algorithm.
Rory Nolan, Luis A J Alvarez, Jonathan Elegheert, Maro Iliopoulou, G Maria Jakobsdottir, Marina Rodriguez-Muñoz, A Radu Aricescu, Sergi Padilla-Parra.
Bioinformatics. 2017-07-11. 33(21) : 3508-3510.
10.1093/bioinformatics/btx434 -
Structural basis for integration of GluD receptors within synaptic organizer complexes.
Jonathan Elegheert, Wataru Kakegawa, Jordan E. Clay, Natalie F. Shanks, Ester Behiels, Keiko Matsuda, Kazuhisa Kohda, Eriko Miura, Maxim Rossmann, Nikolaos Mitakidis, Junko Motohashi, Veronica T. Chang, Christian Siebold, Ingo H. Greger, Terunaga Nakagawa, Michisuke Yuzaki, A. Radu Aricescu.
Science. 2016-07-14. 353(6296) : 295-299.
10.1126/science.aae0104 -
Structure and Assembly Mechanism of the Signaling Complex Mediated by Human CSF-1.
Jan Felix, Steven De Munck, Kenneth Verstraete, Leander Meuris, Nico Callewaert, Jonathan Elegheert, Savvas N. Savvides.
Structure. 2015-09-01. 23(9) : 1621-1631.
10.1016/j.str.2015.06.019 -
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Tao-Hsin Chang, Fu-Lien Hsieh, Matthias Zebisch, Karl Harlos, Jonathan Elegheert, E Yvonne Jones.
eLife. 2015-07-09. 4
10.7554/eLife.06554 -
Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein.
Eleanor G Healey, Benjamin Bishop, Jonathan Elegheert, Christian H Bell, Sergi Padilla-Parra, Christian Siebold.
Nat Struct Mol Biol. 2015-05-04. 22(6) : 458-465.
10.1038/nsmb.3016 -
Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis
Charlotte H. Coles, Nikolaos Mitakidis, Peng Zhang, Jonathan Elegheert, Weixian Lu, Andrew W. Stoker, Terunaga Nakagawa, Ann Marie Craig, E. Yvonne Jones, A. Radu Aricescu.
Nat Commun. 2014-11-11. 5(1)
10.1038/ncomms6209 -
The bacterial antitoxin HipB establishes a ternary complex with operator DNA and phosphorylated toxin HipA to regulate bacterial persistence.
Yurong Wen, Ester Behiels, Jan Felix, Jonathan Elegheert, Bjorn Vergauwen, Bart Devreese, Savvas N. Savvides.
Nucleic Acids Research. 2014-07-23. 42(15) : 10134-10147.
10.1093/nar/gku665 -
Human IL-34 and CSF-1 establish structurally similar extracellular assemblies with their common hematopoietic receptor.
Jan Felix, Jonathan Elegheert, Irina Gutsche, Alexander V. Shkumatov, Yurong Wen, Nathalie Bracke, Erwin Pannecoucke, Isabel Vandenberghe, Bart Devreese, Dmitri I. Svergun, Ewald Pauwels, Bjorn Vergauwen, Savvas N. Savvides.
Structure. 2013-04-01. 21(4) : 528-539.
10.1016/j.str.2013.01.018 -
Allosteric competitive inactivation of hematopoietic CSF-1 signaling by the viral decoy receptor BARF1.
Jonathan Elegheert, Nathalie Bracke, Philippe Pouliot, Irina Gutsche, Alexander V Shkumatov, Nicolas Tarbouriech, Kenneth Verstraete, Anaïs Bekaert, Wim P Burmeister, Dmitri I Svergun, Bart N Lambrecht, Bjorn Vergauwen, Savvas N Savvides.
Nat Struct Mol Biol. 2012-08-19. 19(9) : 938-947.
10.1038/nsmb.2367 -
Extracellular complexes of the hematopoietic human and mouse CSF-1 receptor are driven by common assembly principles.
Jonathan Elegheert, Ambroise Desfosses, Alexander V. Shkumatov, Xiongwu Wu, Nathalie Bracke, Kenneth Verstraete, Kathleen Van Craenenbroeck, Bernard R. Brooks, Dmitri I. Svergun, Bjorn Vergauwen, Irina Gutsche, Savvas N. Savvides.
Structure. 2011-12-01. 19(12) : 1762-1772.
10.1016/j.str.2011.10.012 -
Structural insights into the extracellular assembly of the hematopoietic Flt3 signaling complex.
K. Verstraete, G. Vandriessche, M. Januar, J. Elegheert, A. V. Shkumatov, A. Desfosses, K. Van Craenenbroeck, D. I. Svergun, I. Gutsche, B. Vergauwen, S. N. Savvides.
Blood. 2011-03-09. 118(1) : 60-68.
10.1182/blood-2011-01-329532 -
Inducible production of recombinant human Flt3 ectodomain variants in mammalian cells and preliminary crystallographic analysis of Flt3 ligand-receptor complexes.
Kenneth Verstraete, Bert Remmerie, Jonathan Elegheert, Beatrice Lintermans, Guy Haegeman, Peter Vanhoenacker, Kathleen Van Craenenbroeck, Savvas N. Savvides.
Acta Cryst Sect F. 2011-02-23. 67(3) : 325-331.
10.1107/S1744309111003319 -
Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.
B. Vergauwen, J. Elegheert, A. Dansercoer, B. Devreese, S. N. Savvides.
Proceedings of the National Academy of Sciences. 2010-07-13. 107(30) : 13270-13275.
10.1073/pnas.1005198107 -
Towards structural studies of the old yellow enzyme homologue SYE4 from Shewanella oneidensis and its complexes at atomic resolution.
Jonathan Elegheert, Debbie van den Hemel, Ina Dix, Jan Stout, Jozef Van Beeumen, Ann Brigé, Savvas N. Savvides.
Acta Cryst Sect F. 2009-12-25. 66(1) : 85-90.
10.1107/S1744309109050386