Florian Levet

Publications

• GloBIAS survey results – An insight into the global bioimage analysis community
  Laura R. de la Ballina, Christa G. Walther, Nicholas D. Condon, Alessandro A. Felder, Martin Schätz, Bettina Schmerl, Ko Sugawara, Clara Prats, Anna Klemm, Kota Miura, Paula Sampaio, Christian Tischer, Shuichi Onami, Rocco D'Antuono, Beth A Cimini, Robert Haase, Agustin A. Corbat, Florian Levet.
  Journal of Microscopy. 2026-07-27.
  10.1111/jmi.70153
• Presynaptic actin nanostructures: A reproducibility case study
  Rensu P. Theart, Florian Levet, Paul Hernández‐Herrera, Christophe Leterrier, Laura R. de la Ballina.
  Journal of Microscopy. 2026-07-24.
  10.1111/jmi.70138
• GloBIAS: strengthening the foundations of bioimage analysis
  Agustin A. Corbat, Christa G. Walther, Laura R. de la Ballina, Nicholas D. Condon, Alessandro A. Felder, Martin Schätz, Bettina Schmerl, Ko Sugawara, Clara Prats, Anna Klemm, Kota Miura, Paula Sampaio, Christian Tischer, Florian Levet, Rocco D’Antuono, Beth A. Cimini, Robert Haase.
  Nature Methods. 2026-04-22.
  10.1038/s41592-026-03060-7
• Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis
  Pierre Dupuy, Yves-Marie Boudehen, Marion Faucher, John A Buglino, Allison Fay, Sylvain Cantaloube, Yasmina Grimoire, Julien Marcoux, Florian Levet, Laetitia Bettarel, Bertille Voisin, Jérôme Rech, Jean-Yves Bouet, Olivier Saurel, Jean-Baptiste Sibarita, Michael Glickman, Claude Gutierrez, Olivier Neyrolles.
  The EMBO Journal. 2026-02-13.
  10.1038/s44318-026-00715-1
• In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy
  Marine Cabillic, Hisham Forriere, Laetitia Bettarel, Corey Butler, Abdelghani Neuhaus, Ihssane Idrissi, Miguel Edouardo Sambrano-Lopez, Julian Rossbroich, Lucas-Raphael Müller, Jonas Ries, Gianluca Grenci, Virgile Viasnoff, Florian Levet, Jean-Baptiste Sibarita, Rémi Galland.
  Nat Commun. 2025-09-24. 16(1)
  10.1038/s41467-025-62198-8
• High-affinity detection of biotinylated endogenous neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse
  Charles Ducrot, Adèle Drouet, Béatrice Tessier, Chloé Desquines, Tiffany Cloâtre, Rania-Cérine Mazouzi, Florian Levet, Alexandre Favereaux, Mathieu Letellier, Olivier Thoumine.
  Proc. Natl. Acad. Sci. U.S.A.. 2025-05-27. 122(22)
  10.1073/pnas.2411669122
• Arkitekt: streaming analysis and real-time workflows for microscopy
  Johannes Roos, Stéphane Bancelin, Tom Delaire, Alexander Wilhelmi, Florian Levet, Maren Engelhardt, Virgile Viasnoff, Rémi Galland, U. Valentin Nägerl, Jean-Baptiste Sibarita.
  Nat Methods. 2024-09-18.
  10.1038/s41592-024-02404-5
• Protocol for matching protein localization to synapse morphology in primary rat neurons by correlative super-resolution microscopy
  Tiffany Cloâtre, Magali Mondin, Jean-Baptiste Sibarita, Florian Levet, Olivier Thoumine.
  STAR Protocols. 2024-09-01. 5(3) : 103160.
  10.1016/j.xpro.2024.103160
• Neuroligin-1 dependent phosphotyrosine signaling in excitatory synapse differentiation
  Zsófia Szíber, Adèle Drouet, Magali Mondin, Florian Levet, Olivier Thoumine.
  Front. Mol. Neurosci.. 2024-05-15. 17
  10.3389/fnmol.2024.1359067
• Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens
  Mark Rigby, Federico W. Grillo, Benjamin Compans, Guilherme Neves, Julia Gallinaro, Sophie Nashashibi, Sally Horton, Pedro M. Pereira Machado, Maria Alejandra Carbajal, Gema Vizcay-Barrena, Florian Levet, Jean-Baptiste Sibarita, Angus Kirkland, Roland A. Fleck, Claudia Clopath, Juan Burrone.
  Cell Reports. 2023-05-01. 42(5) : 112397.
  10.1016/j.celrep.2023.112397
• PoCA: a software platform for point cloud data visualization and quantification
  Florian Levet, Jean-Baptiste Sibarita.
  Nat Methods. 2023-03-03.
  10.1038/s41592-023-01811-4
• A framework for evaluating the performance of SMLM cluster analysis algorithms
  Daniel J. Nieves, Jeremy A. Pike, Florian Levet, David J. Williamson, Mohammed Baragilly, Sandra Oloketuyi, Ario de Marco, Juliette Griffié, Daniel Sage, Edward A. K. Cohen, Jean-Baptiste Sibarita, Mike Heilemann, Dylan M. Owen.
  Nat Methods. 2023-02-01. 20(2) : 259-267.
  10.1038/s41592-022-01750-6
• MID3A: Microscopy Image Denoising meets Differentiable Data Augmentation
  Felix Fuentes-Hurtado, Tom Delaire, Florian Levet, Jean-Baptiste Sibarita, Virgile Viasnoff.
  2022 International Joint Conference on Neural Networks (IJCNN). 2022-07-18.
  10.1109/IJCNN55064.2022.9892954
• Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification
  Anne Beghin, Gianluca Grenci, Geetika Sahni, Su Guo, Harini Rajendiran, Tom Delaire, Saburnisha Binte Mohamad Raffi, Damien Blanc, Richard de Mets, Hui Ting Ong, Xareni Galindo, Anais Monet, Vidhyalakshmi Acharya, Victor Racine, Florian Levet, Remi Galland, Jean-Baptiste Sibarita, Virgile Viasnoff.
  Nat Methods. 2022-06-13. 19(7) : 881-892.
  10.1038/s41592-022-01508-0
• A synaptomic analysis reveals dopamine hub synapses in the mouse striatum
  Vincent Paget-Blanc, Marlene E. Pfeffer, Marie Pronot, Paul Lapios, Maria-Florencia Angelo, Roman Walle, Fabrice P. Cordelières, Florian Levet, Stéphane Claverol, Sabrina Lacomme, Mélina Petrel, Christelle Martin, Vincent Pitard, Véronique De Smedt Peyrusse, Thomas Biederer, David Perrais, Pierre Trifilieff, Etienne Herzog.
  Nat Commun. 2022-06-03. 13(1)
  10.1038/s41467-022-30776-9
• Editorial: Methods and Tools for Bioimage Analysis
  Florian Levet, Virginie Uhlmann, Florian Jug.
  Front. Comput. Sci.. 2022-05-30. 4
  10.3389/fcomp.2022.931939
• CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation
  Tomohisa Hosokawa, Pin-Wu Liu, Qixu Cai, Joana S. Ferreira, Florian Levet, Corey Butler, Jean-Baptiste Sibarita, Daniel Choquet, Laurent Groc, Eric Hosy, Mingjie Zhang, Yasunori Hayashi.
  Nat Neurosci. 2021-04-29.
  10.1038/s41593-021-00843-3
• Developing open-source software for bioimage analysis: opportunities and challenges
  Florian Levet, Anne E. Carpenter, Kevin W. Eliceiri, Anna Kreshuk, Peter Bankhead, Robert Haase.
  F1000Res. 2021-04-19. 10 : 302.
  10.12688/f1000research.52531.1
• Distance-dependent regulation of NMDAR nanoscale organization along hippocampal neuron dendrites
  Joana S. Ferreira, Julien P. Dupuis, Blanka Kellermayer, Nathan Bénac, Constance Manso, Delphine Bouchet, Florian Levet, Corey Butler, Jean-Baptiste Sibarita, Laurent Groc.
  Proc Natl Acad Sci USA. 2020-09-14. : 201922477.
  10.1073/pnas.1922477117
• Nanoscale co-organization and coactivation of AMPAR, NMDAR, and mGluR at excitatory synapses
  Julia Goncalves, Tomas M. Bartol, Côme Camus, Florian Levet, Ana Paula Menegolla, Terrence J. Sejnowski, Jean-Baptiste Sibarita, Michel Vivaudou, Daniel Choquet, Eric Hosy.
  Proc Natl Acad Sci USA. 2020-06-08. : 201922563.
  10.1073/pnas.1922563117
• Author Correction: Structural basis of astrocytic Ca2+ signals at tripartite synapses
  Misa Arizono, V. V. G. Krishna Inavalli, Aude Panatier, Thomas Pfeiffer, Julie Angibaud, Florian Levet, Mirelle J. T. Ter Veer, Jillian Stobart, Luigi Bellocchio, Katsuhiko Mikoshiba, Giovanni Marsicano, Bruno Weber, Stéphane H. R. Oliet, U. Valentin Nägerl.
  Nat Commun. 2020-05-18. 11(1)
  10.1038/s41467-020-16453-9
• Structural basis of astrocytic Ca2+ signals at tripartite synapses
  Misa Arizono, V. V. G. Krishna Inavalli, Aude Panatier, Thomas Pfeiffer, Julie Angibaud, Florian Levet, Mirelle J. T. Ter Veer, Jillian Stobart, Luigi Bellocchio, Katsuhiko Mikoshiba, Giovanni Marsicano, Bruno Weber, Stéphane H. R. Oliet, U. Valentin Nägerl.
  Nat Commun. 2020-04-20. 11(1)
  10.1038/s41467-020-15648-4
• SpineJ: A software tool for quantitative analysis of nanoscale spine morphology
  Florian Levet, Jan Tønnesen, U. Valentin Nägerl, Jean-Baptiste Sibarita.
  Methods. 2020-03-01. 174 : 49-55.
  10.1016/j.ymeth.2020.01.020
• Synaptic and supra-synaptic organisation of the dopaminergic projection to the striatum.
  Marlene Pfeffer, Marie Pronot, Maria Florencia Angelo, Roman Walle, Fabrice P Cordelieres, Florient Levet, Stephane Claverol, Sabrina Lacome, Melina Petrel, Christelle Martin, Vincent Pitard, Veronique Desmedt-Peyrusse, Thomas Biederer, Pierre Trifilieff, Etienne Herzog.
  . 2020-02-18.
  10.1101/2020.02.18.952978
• A super-resolution platform for correlative live single-molecule imaging and STED microscopy
  V. V. G. Krishna Inavalli, Martin O. Lenz, Corey Butler, Julie Angibaud, Benjamin Compans, Florian Levet, Jan Tønnesen, Olivier Rossier, Gregory Giannone, Olivier Thoumine, Eric Hosy, Daniel Choquet, Jean-Baptiste Sibarita, U. Valentin Nägerl.
  Nat Methods. 2019-10-21.
  10.1038/s41592-019-0611-8
• SpineJ : A software tool for quantitative analysis of nanoscale spine morphology
  Florian Levet, Jan Tønnesen, Valentin Nägerl, Jean-Baptiste Sibarita.
  . 2019-09-10.
  10.1101/764548
• Differential role of pre- and postsynaptic neurons in the activity-dependent control of synaptic strengths across dendrites
  Mathieu Letellier, Florian Levet, Olivier Thoumine, Yukiko Goda.
  PLoS Biol. 2019-06-05. 17(6) : e2006223.
  10.1371/journal.pbio.2006223
• A tessellation-based colocalization analysis approach for single-molecule localization microscopy
  Florian Levet, Guillaume Julien, Rémi Galland, Corey Butler, Anne Beghin, Anaël Chazeau, Philipp Hoess, Jonas Ries, Grégory Giannone, Jean-Baptiste Sibarita.
  Nat Commun. 2019-05-30. 10(1)
  10.1038/s41467-019-10007-4
• A tessellation-based colocalization analysis approach for single-molecule localization microscopy
  Florian Levet, Guillaume Julien, Rémi Galland, Corey Butler, Anne Beghin, Anaël Chazeau, Philipp Hoess, Jonas Ries, Grégory Giannone, Jean-Baptiste Sibarita.
  Nat Commun. 2019-05-30. 10(1)
  10.1038/s41467-019-10007-4
• Differential Nanoscale Topography and Functional Role of GluN2-NMDA Receptor Subtypes at Glutamatergic Synapses
  Blanka Kellermayer, Joana S. Ferreira, Julien Dupuis, Florian Levet, Dolors Grillo-Bosch, Lucie Bard, Jeanne Linarès-Loyez, Delphine Bouchet, Daniel Choquet, Dmitri A. Rusakov, Pierre Bon, Jean-Baptiste Sibarita, Laurent Cognet, Matthieu Sainlos, Ana Luisa Carvalho, Laurent Groc.
  Neuron. 2018-10-01. 100(1) : 106-119.e7.
  10.1016/j.neuron.2018.09.012
• Fast Sampling of Implicit Surfaces by Particle Systems
  F. Levet, X. Granier, C. Schlick.
  IEEE International Conference on Shape Modeling and Applications 2006 (SMI'06). .
  10.1109/smi.2006.13
• Multi-view Sketch-Based FreeForm Modeling
  Florian Levet, Xavier Granier, Christophe Schlick.
  Smart Graphics. . : 204-209.
  10.1007/978-3-540-73214-3_21
• Thor: Sketch-Based 3D Modeling by Skeletons
  Romain Arcila, Florian Levet, Christophe Schlick.
  Smart Graphics. . : 232-238.
  10.1007/978-3-540-85412-8_22
• Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
  Tomáš Lukeš, Daniela Glatzová, Zuzana Kvíčalová, Florian Levet, Aleš Benda, Sebastian Letschert, Markus Sauer, Tomáš Brdička, Theo Lasser, Marek Cebecauer.
  Nat Commun. 2017-11-23. 8(1)
  10.1038/s41467-017-01857-x
• Localization-based super-resolution imaging meets high-content screening
  Anne Beghin, Adel Kechkar, Corey Butler, Florian Levet, Marine Cabillic, Olivier Rossier, Gregory Giannone, Rémi Galland, Daniel Choquet, Jean-Baptiste Sibarita.
  Nat Meth. 2017-10-30. 14(12) : 1184-1190.
  10.1038/nmeth.4486
• Nanoscale organization of synaptic adhesion proteins revealed by single-molecule localization microscopy
  Ingrid Chamma, Florian Levet, Jean-Baptiste Sibarita, Matthieu Sainlos, Olivier Thoumine.
  Neurophoton. 2016-11-03. 3(4) : 041810.
  10.1117/1.NPh.3.4.041810
• SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data.
  Florian Levet, Eric Hosy, Adel Kechkar, Corey Butler, Anne Beghin, Daniel Choquet, Jean-Baptiste Sibarita.
  Nat Methods. 2015-09-07. 12(11) : 1065-1071.
  10.1038/nmeth.3579
• Frequency of Cocaine Self-Administration Influences Drug Seeking in the Rat: Optogenetic Evidence for a Role of the Prelimbic Cortex
  Elena Martín-García, Julien Courtin, Prisca Renault, Jean- François Fiancette, Hélène Wurtz, Amélie Simonnet, Florian Levet, Cyril Herry, Véronique Deroche-Gamonet.
  Neuropsychopharmacol. 2014-03-17. 39(10) : 2317-2330.
  10.1038/npp.2014.66
• Target-specific vulnerability of excitatory synapses leads to deficits in associative memory in a model of intellectual disorder
  X. Houbaert, C.-L. Zhang, F. Gambino, M. Lepleux, M. Deshors, E. Normand, F. Levet, M. Ramos, P. Billuart, J. Chelly, E. Herzog, Y. Humeau.
  Journal of Neuroscience. 2013-08-21. 33(34) : 13805-13819.
  10.1523/JNEUROSCI.1457-13.2013
• Neurexin-Neuroligin Adhesions Capture Surface-Diffusing AMPA Receptors through PSD-95 Scaffolds
  M. Mondin, V. Labrousse, E. Hosy, M. Heine, B. Tessier, F. Levet, C. Poujol, C. Blanchet, D. Choquet, O. Thoumine.
  Journal of Neuroscience. 2011-09-21. 31(38) : 13500-13515.
  10.1523/jneurosci.6439-10.2011
• Neurexin-neuroligin adhesions capture surface-diffusing AMPA receptors through PSD-95 scaffolds.
  M. Mondin, V. Labrousse, E. Hosy, M. Heine, B. Tessier, F. Levet, C. Poujol, C. Blanchet, D. Choquet, O. Thoumine.
  Journal of Neuroscience. 2011-09-21. 31(38) : 13500-13515.
  10.1523/jneurosci.6439-10.2011
• Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density.
  Gregory Giannone, Eric Hosy, Florian Levet, Audrey Constals, Katrin Schulze, Alexander I. Sobolevsky, Michael P. Rosconi, Eric Gouaux, Robert Tampé, Daniel Choquet, Laurent Cognet.
  Biophysical Journal. 2010-08-01. 99(4) : 1303-1310.
  10.1016/j.bpj.2010.06.005
• Improved skeleton extraction and surface generation for sketch-based modeling
  Florian Levet, Xavier Granier.
  Proceedings of Graphics Interface 2007 on - GI '07. 2007-01-01.
  10.1145/1268517.1268524
• 3D Sketching with Profile Curves
  Florian Levet, Xavier Granier, Christophe Schlick.
  Smart Graphics. 2006-01-01. : 114-125.
  10.1007/11795018_11