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Thèse – Chloè Lacabanne

17 décembre 2018 / 00:00

Inflammation and immune-mediated neurobehavioral alterations: a critical role for microglia

Thèse en co-tutelle Université de Bordeaux-McGill University Sous la co-direction de Sophie Layé (NutriNeuro, Bordeaux) et Giamal Luheshi (Department of Psychiatry, McGill, Montréal)

Lieu et date de la soutenance : McGill University, Montréal, 17 décembre 2018

Recent research on microglia has uncovered a multitude of activities that extends the role of these cells well beyond their traditional function as immune sentinels. The most prominent of these newly described activities is an intricate role in neuronal network remodeling notably upon environmental challenge or during brain development, the disruption of which can result in long lasting consequences relevant to several psychopathologies. We sought, in the current thesis, to identify some of the mechanisms involved. First, we targeted the immune function of microglia, based on our previous findings linking systemic immunogenic challenge with lipopolysaccharide (LPS) in mice with the development of despair-like behaviour/depression. These studies were conducted in adult mice in which phagocytic microglia were depleted using a single injection of liposomal clodronate in the CA3 region of the hippocampus. Clodronate-mediated depletion attenuated most of the LPS-induced cytokine expression in the hippocampus and -depressive-like symptoms, providing a snapshot of the role of inflammation in the development of brain dysfunction mediated by microglia. In subsequent studies, we investigated the role of microglia activity in prenatal LPS-induced neurodevelopmental disorders.
Our results demonstrated maternal LPS administration reduced the percentage of mature microglial population in the brain of embryos and impaired the offspring’s neonatal as well as adult social and repetitive behavior and memory, with a clear divergence along sex lines in adulthood. We then identified the role played by pro-inflammatory cytokines, particularly IL-1β in LPS-induced impairment of microglia in the brain of embryos in complex behaviours. In conclusion, the work presented in this thesis shows the role of microglia in mediating short- and long-term LPS-induced behavioural deficits.

Notably, our results show that depletion of phagocytic microglia can attenuate LPS-induced despair-like behaviours while LPS-induced inflammation during embryonic development leads to the exhibition of sexually dimorphic traits and disrupted microglial development for which IL-1β appears to be the primary mediator.

Publications

Neuroinflammation in Autism: Plausible Role of Maternal Inflammation, Dietary Omega 3, and Microbiota Charlotte Madore, Quentin Leyrolle, Chloé Lacabanne, Anouk Benmamar–Badel, Corinne Joffre, Agnès Nadjar and Sophie Layé (Published. Neural Plasticity, 2016 October • 2016:3597209

Microglial Activation Enhances Associative Taste Memory through Purinergic Modulation of Glutamatergic Neurotransmission Jean-Christophe Delpech, Nicolas Saucisse, Shauna L. Parkes, Chloé Lacabanne, Agnès Aubert, Fabrice Casenave, Etienne Coutureau, Nathalie Sans, Sophie Layé, Guillaume Ferreira* and Agnès Nadjar* (Published. The journal of Neuroscience, 2015 February • 35(7):3022–3033)

Dietary n-3 PUFAs Deficiency Increases Vulnerability to Inflammation-Induced Spatial Memory Impairment Jean-Christophe Delpech, Aurore Thomazeau, Charlotte Madore, Clémentine Bosch-Bouju, Thomas Larrieu, Chloé Lacabanne, Julie Remus-Borel, Agnès Aubert, Corine Joffre, Agnès Nadjar and Sophie Layé (Published. Neuropsychopharmacology, 2015 November • 40(12):2774-87)

Interleukin-1β mediates the effects of prenatal LPS induced behavioral anomalies in the offspring through modulating microglia Chloé Lacabanne, Lourdes Fernandez de Cossio, Sophie Layé* and Giamal N. Luheshi* (In preparation)

 

JURY

Pr Pierre Gressens, Directeur de Recherche Inserm, Paris, France
Pr Lalit Srivastava, Full Professor, McGill University, Montréal, Canada
Dr Steve Lacroix, Research Director, Université Laval, Québec, Canada
Dr Thierry Alquier, Research Director, Université de Montréal, Montréal, Canada
Dr David Gosselin, Research Director, Université Laval, Québec, Canada
Dr Sophie Layé, Directrice de Recherche Inra, Bordeaux, France
Pr Giamal Luheshi, Full professor, McGill University, Montréal, Canada
Pr Josephine Nalbantoglu, Full Professor, McGill University, Montréal, Canada

Prochainement

  1. Séminaire – David Stellwagen

    jeudi 20 juin 2019 / 15:30

    TNF-mediated synaptic plasticity in the response to stress and reward

  2. Séminaire – André Ramos

    vendredi 21 juin 2019 / 11:30

    The long (and uncertain) way from anxiety-related behaviors to genes

  3. Séminaire – Manojkumar Saranathan

    vendredi 21 juin 2019 / 14:30

    Segmentation of thalamic nuclei from 3T MRI with applications

  4. Cajal course : Biosensors and actuators for cellular and systems neuroscience

    dimanche 23 juin 2019 - samedi 13 juillet 2019

    Les séminaires sont ouverts à tous.

  5. Soutenance de thèse – Sandra Dubes

    lundi 24 juin 2019 / 14:30

    Rôle du microARN miR-124 dans la plasticité homéostatique via le contrôle de l’expression de la synaptopodine et des récepteurs AMPA dans les neurones de l'hippocampe.

Détails

Date :
17 décembre 2018
Heure :
00:00
Catégorie d’Évènement: