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Séminaire – Stefan Hallermann

vendredi 12 juillet 2019 / 11:30

Large, invariable action potential amplitude in neocortical nerve terminals revealed by high-resolution current-clamp recordings

Lieu : Centre Broca Nouvelle Aquitaine


Stefan Hallermann
Carl-Ludwig-Institute for Physiology
University Leipzig – Germany
http://physiologie.medizin.uni-leipzig.de/

 

Invité par Christophe Mulle

 

Abstract

The presynaptic action potential critically controls synaptic transmission. At experimentally- accessible large nerve terminals, sodium and potassium channels control the amplitude and duration of action potentials, respectively. Recently, potassium channels were found to dynamically regulate action potential amplitude in small nerve terminals and this effect was proposed as a novel mechanism for synaptic plasticity. To investigate this apparent difference between large and small terminals, we established high-resolution current-clamp recordings from small nerve terminals of cultured neocortical neurons. Using an electrical equivalent circuit and quartz glass pipettes, we systematically investigated errors related to pipette capacitance and the performance of current-clamp amplifiers. We found rapid action potentials with an amplitude of ~120 mV. Blocking potassium channels prolonged action potentials but did not affect their amplitude. Furthermore, the spike amplitude was unaffected during short-term and homeostatic plasticity. Thus, our data indicate large and stable action potential amplitude at small neocortical nerve terminals.

Selected publications

Haselmann H, Mannara F, Werner C, Planagumà J, Miguez-Cabello F, Schmidl L, Grünewald B, Petit-Pedrol M, Kirmse K, Classen J, Demir F, Klöcker N, Soto D, Doose S, Dalmau J, Hallermann S, Geis C (2018) Human Autoantibodies against the AMPA Receptor Subunit GluA2 Induce Receptor Reorganization and Memory Dysfunction. Neuron 100:91-105

Ritzau-Jost A, Jablonski L, Viotti J, Lipstein N, Eilers J, and Hallermann S. (2018) Apparent calcium dependence of vesicle recruitment. J Physiol 596:4693-707

Delvendahl I, Vyleta NP, von Gersdorff H, and Hallermann S. (2016) Fast, temperature-sensitive and clathrin-independent endocytosis at central synapses. Neuron 90:492-8

Delvendahl I, Jablonski L, Baade C, Matveev V, Neher E, and Hallermann S. (2015) Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling. Proc Natl Acad Sci U S A 112:E3075-84

Ritzau-Jost A, Delvendahl I, Rings A, Byczkowicz N, Harada H, Shigemoto R, Hirrlinger J, Eilers J, and Hallermann S. (2014) Ultrafast action potentials mediate kilohertz signaling at a central synapse. Neuron 84:152-63

Prochainement

  1. Séminaire – Tomonori Takeuchi

    lundi 17 juin 2019 / 11:30

    Memory consolidation, dopamine and two distinct novelty systems

  2. Séminaire – David Stellwagen

    jeudi 20 juin 2019 / 15:30

    TNF-mediated synaptic plasticity in the response to stress and reward

  3. Séminaire – André Ramos

    vendredi 21 juin 2019 / 11:30

    Le long (et incertain) chemin entre les comportements liés à l'anxiété et les gènes sous-jacents

  4. Séminaire – Manojkumar Saranathan

    vendredi 21 juin 2019 / 14:30

    Segmentation of thalamic nuclei from 3T MRI with applications

  5. Cajal course : Biosensors and actuators for cellular and systems neuroscience

    dimanche 23 juin 2019 - samedi 13 juillet 2019

    Les séminaires sont ouverts à tous.

Détails

Date :
vendredi 12 juillet 2019
Heure :
11:30
Catégorie d’Évènement: