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Séminaire – Stefan Hallermann

vendredi 12 juillet 2019 / 11:30

Large, invariable action potential amplitude in neocortical nerve terminals revealed by high-resolution current-clamp recordings

Lieu : Centre Broca Nouvelle Aquitaine


Stefan Hallermann
Carl-Ludwig-Institute for Physiology
University Leipzig – Germany
http://physiologie.medizin.uni-leipzig.de/

 

Invité par Christophe Mulle

 

Abstract

The presynaptic action potential critically controls synaptic transmission. At experimentally- accessible large nerve terminals, sodium and potassium channels control the amplitude and duration of action potentials, respectively. Recently, potassium channels were found to dynamically regulate action potential amplitude in small nerve terminals and this effect was proposed as a novel mechanism for synaptic plasticity. To investigate this apparent difference between large and small terminals, we established high-resolution current-clamp recordings from small nerve terminals of cultured neocortical neurons. Using an electrical equivalent circuit and quartz glass pipettes, we systematically investigated errors related to pipette capacitance and the performance of current-clamp amplifiers. We found rapid action potentials with an amplitude of ~120 mV. Blocking potassium channels prolonged action potentials but did not affect their amplitude. Furthermore, the spike amplitude was unaffected during short-term and homeostatic plasticity. Thus, our data indicate large and stable action potential amplitude at small neocortical nerve terminals.

Selected publications

Haselmann H, Mannara F, Werner C, Planagumà J, Miguez-Cabello F, Schmidl L, Grünewald B, Petit-Pedrol M, Kirmse K, Classen J, Demir F, Klöcker N, Soto D, Doose S, Dalmau J, Hallermann S, Geis C (2018) Human Autoantibodies against the AMPA Receptor Subunit GluA2 Induce Receptor Reorganization and Memory Dysfunction. Neuron 100:91-105

Ritzau-Jost A, Jablonski L, Viotti J, Lipstein N, Eilers J, and Hallermann S. (2018) Apparent calcium dependence of vesicle recruitment. J Physiol 596:4693-707

Delvendahl I, Vyleta NP, von Gersdorff H, and Hallermann S. (2016) Fast, temperature-sensitive and clathrin-independent endocytosis at central synapses. Neuron 90:492-8

Delvendahl I, Jablonski L, Baade C, Matveev V, Neher E, and Hallermann S. (2015) Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling. Proc Natl Acad Sci U S A 112:E3075-84

Ritzau-Jost A, Delvendahl I, Rings A, Byczkowicz N, Harada H, Shigemoto R, Hirrlinger J, Eilers J, and Hallermann S. (2014) Ultrafast action potentials mediate kilohertz signaling at a central synapse. Neuron 84:152-63

Prochainement

  1. Conférence mensuelle (PhD seminar series) – Laurent Bourdieu

    vendredi 6 septembre 2019 / 11:30

    Fast 3D imaging of neuronal activity in visual cortex across multiple layers in awake mice

  2. Cajal lectures : Whole brain imaging

    lundi 9 septembre 2019 - vendredi 27 septembre 2019

    Free entrance lectures / Venue: CGFB

  3. Séminaire – Francisco Papaleo

    vendredi 13 septembre 2019 / 11:30

    Bottom-Up and Top-Down Control of Emotion Recognition.

  4. LabEx TRAIL scientific day

    lundi 16 septembre 2019 / 14:00 - 18:00

    Dedicated to brain connectivity.

  5. Journée Synapse

    mardi 17 septembre 2019

    Centre Broca Nouvelle-Aquitaine


Conférence internationale : REWARD

Du 2 au 4 octobre

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Détails

Date :
vendredi 12 juillet 2019
Heure :
11:30
Catégorie d’Évènement: