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Jakob Balslev Sørensen
Department of Neuroscience,
University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Using neuroendocrine cells to study the role of synaptotagmin-7 in vesicle priming and fusion.
Fusion of dense-core or synaptic vesicles with the plasma membrane is the final step in the exocytotic cascade leading to release of neurotransmitter and neuropeptides. The penultimate step is vesicle priming: the build-up of a fusion machinery between the vesicle and the plasma membrane. There is amble evidence that regulation of priming is a key element in presynaptic and secretory plasticity, however distinguishing between vesicle priming and fusion is often difficult, because typically only the final step (neurotransmitter release) is directly measured. Here, I will discuss how we use adrenal chromaffin cells, capacitance measurements, calcium uncaging and 3D Electron Tomography to dissect effects on vesicle priming and fusion. I will show how we recently used this powerful system to describe the function of synaptotagmin-7 in vesicle priming and fusion.
Invited by Bordeaux Neurocampus and the NBA