Genotyping

Faced with the increasing use of transgenic models, Neurocentre Magendie developed in 2008 a unique genotyping service aimed at characterizing the genetic modifications of these models. This department, originally created through the sharing of human skills and material resources, has over the years developed automated and standardized methods for fast and reliable analyses. For more than 15 years, the platform has been developing its expertise and now proposes more than 400 PCR, qPCR or RFLP protocols, for an analysis of more than 1000 transgenic models. Today, a team of 4 experts is mobilized to meet the expectations of the scientific community. More than 60,000 samples are processed each year to meet the growing demand of many research institutes in France.

Beyond genotyping, the platform also provides a service to verify the absence of mycoplasma in cell culture media, thereby helping to ensure the quality and reliability of experimental work. It also supports Neurocentre teams in their projects involving the import of new lines and manages the establishment of breeding according to experimental needs. In collaboration with the Neurocentre bioinformatics platform, the genotyping platform has developed a secure web application, ANISE, which enables production monitoring and provides real-time visibility of transgenic lines while meeting regulatory requirements.