Two-dimensional blue native/SDS gel electrophoresis of multiprotein complexes

Jean Paul Lasserre, Armelle Ménard
Methods in Molecular Biology. 2012-01-01; : 317-337
DOI: 10.1007/978-1-61779-821-4_27

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1. Methods Mol Biol. 2012;869:317-37. doi: 10.1007/978-1-61779-821-4_27.

Two-dimensional blue native/SDS gel electrophoresis of multiprotein complexes.

Lasserre JP(1), Ménard A.

Author information:
(1)Laboratoire de Bactériologie, INSERM U853, Université Victor Segalen Bordeaux
2, Bordeaux Cedex, France.

The two-dimensional blue native/sodium dodecyl sulfate polyacrylamide gel
electrophoresis (2D BN/SDS-PAGE) is a method of choice for the investigation of
protein complexes. This highly resolvent separation method is unique in that it
facilitates the identification of many protein complexes simultaneously. Because
of its simplicity and suitability, the 2D BN/SDS-PAGE can be now applied to a
wide range of organisms, including bacteria, viruses, yeasts, animals, and
plants. Moreover, recent modifications have made it possible to apply this method
to the study of whole protein complexes of an organism. Here, we propose
protocols for the investigation of the whole complexome of bacteria as well as
eukaryotic cells.

DOI: 10.1007/978-1-61779-821-4_27
PMID: 22585498 [Indexed for MEDLINE]

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