TRPP2 modulates ryanodine- and inositol-1,4,5-trisphosphate receptors-dependent Ca2+ signals in opposite ways in cerebral arteries
Cell Calcium. 2015-11-01; 58(5): 467-475
DOI: 10.1016/j.ceca.2015.07.003
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1. Cell Calcium. 2015 Nov;58(5):467-75. doi: 10.1016/j.ceca.2015.07.003. Epub 2015
Jul 30.
TRPP2 modulates ryanodine- and inositol-1,4,5-trisphosphate receptors-dependent
Ca2+ signals in opposite ways in cerebral arteries.
Abdi A(1), Mazzocco C(2), Légeron FP(1), Yvert B(3), Macrez N(1), Morel JL(4).
Author information:
(1)Univ. Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, F-33000
Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293,
F-33000 Bordeaux, France.
(2)ImmuSmol PTIB, Hopital Xavier Arnozan, Avenue du Haut Leveque, F-33600 Pessac,
France.
(3)INSERM, UA01, Clinatec Laboratory, Biomedical Research Center Edmond J. Safra,
F-38000 Grenoble, France; UJF, UA01, Clinatec Laboratory, Biomedical Research
Center Edmond J. Safra, F-38000 Grenoble, France; CHU Grenoble, UA01, Clinatec
Laboratory, Biomedical Research Center Edmond J. Safra, F-38000 Grenoble, France;
CEA, LETI, Clinatec, Biomedical Research Center Edmond J. Safra, F-38000
Grenoble, France.
(4)Univ. Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, F-33000
Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293,
F-33000 Bordeaux, France. Electronic address: .
TRPP2 is a cationic channel expressed in plasma membrane and in sarcoplasmic
reticulum. In several cell lines, TRPP2 is described as a reticulum Ca(2+) leak
channel but it also interacts with ryanodine and inositol 1,4,5-trisphosphate
(InsP3) receptors to inhibit and increase the release of Ca(2+) stores,
respectively. TRPP2 is known to be expressed in vascular smooth muscle cells,
however its function in Ca(2+) signals remains poorly described in native cells,
principally because the pharmacology is not developed. TRPP2 was expressed in
cerebral arteries. Triptolide evoked Ca(2+) responses in a Ca(2+)-free solution
as well as permeabilized arteries. This Ca(2+) signal was inhibited in presence
of antisense oligonucleotide and siRNA directed against TRPP2 and antibody
directed against the first loop of TRPP2. The partial inhibition of TRPP2
expression increased both the caffeine-evoked Ca(2+) responses and in vivo
contraction. It also decreased the InsP3-evoked Ca(2+) responses. Finally, aging
affected the regulations in which TRPP2 is engaged, whereas the triptolide-evoked
Ca(2+) response was not modified. Taken together, our results have shown that
TRPP2 is implicated in triptolide-induced Ca(2+) release from intracellular
Ca(2+) stores. TRPP2 functionally interacts with both ryanodine and InsP3
receptors. These interactions were not similar in adult and old mice.
Copyright © 2015 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.ceca.2015.07.003
PMID: 26254047 [Indexed for MEDLINE]