The power of next-generation sequencing as illustrated by the neuropeptidome of the crayfish Procambarus clarkii
General and Comparative Endocrinology. 2015-12-01; 224: 84-95
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(1)INCIA UMR 5287 CNRS, Université de Bordeaux, Pessac, France. Electronic
Transcriptomes of the crayfish Procambarus clarkii were analyzed for the presence
of transcripts encoding neurohormones, neuropeptides and their receptors. A total
of 58 different transcripts were found to encode such ligands and another 82 for
their receptors. A very large number of the neuropeptide transcripts appeared to
be complete and for those that were not only small parts seemed to be lacking.
Transcripts for the neuropeptide GPCRs as well as for the putative receptors for
insulin, neuroparsin and eclosion hormone were often also complete or almost so.
Of particular interest is the presence of three different neuroparsin genes and
two putative neuroparsin receptors. There are also three pigment dispersing
hormones as well three likely receptors for these neuropeptides. CNMamide,
calcitonin, CCRFamide, natalisin, trissin and relaxin appear to be new crustacean
neuropeptides. The recently identified crustacean female sex hormone was also
found and in the crayfish appears to be not only expressed in the eyestalk, but
in the ovary as well (though not in the testis). Interestingly, there are two
other proteins in the crayfish with a structure similar to crustacean female sex
hormone, that could be precursors of neurohormones, but these are not expressed
by the ovary. The ovary also appears to contain significant numbers of
transcripts encoding pigment dispersing hormones, CNMamide as well as
glycoprotein B5, but not glycoprotein A2.
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