The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking
Experimental Cell Research. 2016-04-01; 343(1): 28-34
DOI: 10.1016/j.yexcr.2015.11.004
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1. Exp Cell Res. 2016 Apr 10;343(1):28-34. doi: 10.1016/j.yexcr.2015.11.004. Epub
2015 Nov 10.
The journey of integrins and partners in a complex interactions landscape studied
by super-resolution microscopy and single protein tracking.
Rossier O(1), Giannone G(1).
Author information:
(1)Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297,
F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR
5297, F-33000 Bordeaux, France.
Cells adjust their adhesive and cytoskeletal organizations according to changes
in the biochemical and physical nature of their surroundings. In return, by
adhering and generating forces on the extracellular matrix (ECM) cells organize
their microenvironment. Integrin-dependent focal adhesions (FAs) are the
converging zones integrating biochemical and biomechanical signals arising from
the ECM and the actin cytoskeleton. Thus, integrin-mediated adhesion and
mechanotransduction, the conversion of mechanical forces into biochemical
signals, are involved in critical cellular functions such as migration,
proliferation and differentiation, and their deregulation contributes to
pathologies including cancer. A challenging problem is to decipher how stochastic
protein movements and interactions lead to formation of dynamic architecture such
as integrin-dependent adhesive structures. In this review, we will describe
recent advances made possible by super-resolution microscopies and single
molecule tracking approaches that provided new understanding on the organization
and the dynamics of integrins and intracellular regulators at the nanoscale in
living cells.
Copyright © 2015. Published by Elsevier Inc.
DOI: 10.1016/j.yexcr.2015.11.004
PMID: 26571074 [Indexed for MEDLINE]