The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions

Zuccolo M, Alves A, Galy V, Bolhy S, Formstecher E, Racine V, Sibarita JB, Fukagawa T, Shiekhattar R, Yen T, Doye V.
EMBO J.. 2007 Apr 4; 26(7): 1853-64
DOI: 7601642 [pii]10.1038/sj.emboj.7601642

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We previously demonstrated that a fraction of the human Nup107-160 nuclear pore
subcomplex is recruited to kinetochores at the onset of mitosis. However, the
molecular determinants for its kinetochore targeting and the functional
significance of this localization were not investigated. Here, we show that the
Nup107-160 complex interacts with CENP-F, but that CENP-F only moderately
contributes to its targeting to kinetochores. In addition, we show that the
recruitment of the Nup107-160 complex to kinetochores mainly depends on the Ndc80
complex. We further demonstrate that efficient depletion of the Nup107-160
complex from kinetochores, achieved either by combining siRNAs targeting several
of its subunits excluding Seh1, or by depleting Seh1 alone, induces a mitotic
delay. Further analysis of Seh1-depleted cells revealed impaired chromosome
congression, reduced kinetochore tension and kinetochore-microtubule attachment
defects. Finally, we show that the presence of the Nup107-160 complex at
kinetochores is required for the recruitment of Crm1 and RanGAP1-RanBP2 to these
structures. Together, our data thus provide the first molecular clues underlying
the function of the human Nup107-160 complex at kinetochores.

DOI: 10.1038/sj.emboj.7601642
PMCID: PMC1847668
PMID: 17363900

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