The cellular pathway of CD1e in immature and maturing dendritic cells

Angénieux C, Fraisier V, Maître B, Racine V, van der Wel N, Fricker D, Proamer F, Sachse M, Cazenave JP, Peters P, Goud B, Hanau D, Sibarita JB, Salamero J, de la Salle H.
Traffic. 2005 Apr; 6(4): 286-302
DOI: TRA272 [pii]10.1111/j.1600-0854.2005.00272.x

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Dendritic cells (DCs) present antigens to T cells via CD1, HLA class I or class
II molecules. During maturation, HLA class II-restricted presentation is
optimized. The relocalization of CD1e from Golgi to endosomal compartments during
DC maturation suggests also an optimization of the antigen-presentation pathway
via CD1 molecules. We here detail the biosynthesis and cellular pathway of CD1e
in immature and maturing DCs. Unlike the other CD1 molecules, CD1e was found to
reach late endosomes through sorting endosomes, without passing through the
plasma membrane in either immature or maturing cells. After induction of DC
maturation, CD1e disappeared rapidly from the Golgi and was transiently localized
in HLA-DR+ vesicles, while the number of CD1e+/CD1b+ compartments increased for
at least 20 h. High-resolution light microscopy showed that, in immature DCs,
CD1e+ vesicles were often in close apposition to EEA1+ or HLA-DR+ compartments,
while CD1e displayed a nearly exclusive distribution in the lysosomes of mature
DCs, a finding corroborated by immunoelectron microscopy. During maturation, CD1e
synthesis progressively declined, while the endosomal cleavage of CD1e still
occurred. Thus, CD1e displays peculiar properties, suggesting an unexpected role
among the family of CD1 antigen-presenting molecules.

DOI: 10.1111/j.1600-0854.2005.00272.x
PMID: 15752135 [Indexed for MEDLINE]

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