Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation.
J Cell Biol. 2003-10-27; 163(2): 409-419
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1. J Cell Biol. 2003 Oct 27;163(2):409-19.
Talin1 is critical for force-dependent reinforcement of initial
integrin-cytoskeleton bonds but not tyrosine kinase activation.
Giannone G(1), Jiang G, Sutton DH, Critchley DR, Sheetz MP.
(1)Department of Biological Sciences, Columbia University, New York, NY 10027,
Cells rapidly transduce forces exerted on extracellular matrix contacts into
tyrosine kinase activation and recruitment of cytoskeletal proteins to reinforce
integrin-cytoskeleton connections and initiate adhesion site formation. The
relationship between these two processes has not been defined, particularly at
the submicrometer level. Using talin1-deficient cells, it appears that talin1 is
critical for building early mechanical linkages. Deletion of talin1 blocked laser
tweezers, force-dependent reinforcement of submicrometer fibronectin-coated beads
and early formation of adhesion sites in response to force, even though Src
family kinases, focal adhesion kinase, and spreading were activated normally.
Recruitment of vinculin and paxillin to sites of force application also required
talin1. FilaminA had a secondary role in strengthening
fibronectin-integrin-cytoskeleton connections and no role in stretch-dependent
adhesion site assembly. Thus, force-dependent activation of tyrosine kinases is
independent of early force-dependent structural changes that require talin1 as
part of a critical scaffold.
PMID: 14581461 [Indexed for MEDLINE]