Stress downregulates lipopolysaccharide-induced expression of proinflammatory cytokines in the spleen, pituitary, and brain of mice
Brain, Behavior, and Immunity. 1995-12-01; 9(4): 292-303
DOI: 10.1006/brbi.1995.1028
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1. Brain Behav Immun. 1995 Dec;9(4):292-303.
Stress downregulates lipopolysaccharide-induced expression of proinflammatory
cytokines in the spleen, pituitary, and brain of mice.
Goujon E(1), Parnet P, Laye S, Combe C, Kelley KW, Dantzer R.
Author information:
(1)INRA-INSERM, Bordeaux, France.
Mice injected with LPS (10 mu g/mouse, sc) or saline were submitted to a 15-min
restraint stress and sacrificed 1 or 2 h later to assess the effect of stress on
the induction of interleukin-1beta (IL-1beta) and other proinflammatory cytokines
(IL-1alpha, IL-1ra, IL-6, and tumor necrosis factor-alpha) in the spleen,
pituitary, hypothalamus, hippocampus, and striatum. LPS-induced cytokine gene
expression, as determined by comparative RT-PCR, was lower in stressed than in
nonstressed mice. LPS increased plasma and tissue levels of IL-1beta, as
determined by ELISA, but this effect was less marked in stressed than in
nonstressed mice. These results are discussed in relation to the modulatory
effects of glucocorticoids on cytokine production.
DOI: 10.1006/brbi.1995.1028
PMID: 8903847 [Indexed for MEDLINE]