Spontaneously hypertensive rats are highly vulnerable to AMPA-induced brain lesions
Stroke. 2007-09-27; 38(11): 3007-3015
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1. Stroke. 2007 Nov;38(11):3007-15. Epub 2007 Sep 27.
Spontaneously hypertensive rats are highly vulnerable to AMPA-induced brain
Lecrux C(1), Nicole O, Chazalviel L, Catone C, Chuquet J, MacKenzie ET, Touzani
(1)University of Caen, CNRS UMR 6185, Cyceron, Caen, France.
BACKGROUND AND PURPOSE: Whereas the effects of chronic arterial hypertension on
the cerebral vasculature have been widely studied, its effects on brain tissue
have been studied less so. Here we examined if spontaneously hypertensive rats
(SHRs) or the normotensive control Wistar Kyoto rats (WKYs) made hypertensive by
renal artery stenosis (R-WKYs) are vulnerable to an excitotoxic brain lesion
provoked by an overactivation of glutamate receptors.
METHODS: Lesion volumes were quantified by histology in WKYs and SHRs subjected
to striatal administration of N-methyl-d-aspartate (NMDA) or
alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA). The expression
of AMPA receptors subunits and calcium/calmodulin kinase-II alpha was analyzed by
real-time polymerase chain reaction and Western blot.
RESULTS: NMDA (50 and 75 nmol) induced similar lesions in both SHRs (10+/-2 mm(3)
and 16+/-4 mm(3), respectively) and WKYs (11+/-2 mm(3) and 19+/-7 mm(3),
respectively). However, AMPA-induced (2.5 and 5 nmol) lesions were significantly
greater in 14-week-old SHRs (14+/-3 mm(3) and 20+/-5 mm(3), respectively) than
WKYs (4+/-2 mm(3), P