Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation.
Cell Reports. 2017-02-01; 18(8): 1840-1847
DOI: 10.1016/j.celrep.2017.01.081
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Rosendale M(1), Jullié D(1), Choquet D(1), Perrais D(2).
Author information:
(1)University of Bordeaux, 33000 Bordeaux, France; Centre National de la
Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297,
33000 Bordeaux, France.
(2)University of Bordeaux, 33000 Bordeaux, France; Centre National de la
Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297,
33000 Bordeaux, France. Electronic address: .
Endocytosis in neuronal dendrites is known to play a critical role in synaptic
transmission and plasticity such as long-term depression (LTD). However, the
inability to detect endocytosis directly in living neurons has hampered studies
of its dynamics and regulation. Here, we visualized the formation of individual
endocytic vesicles containing pHluorin-tagged receptors with high temporal
resolution in the dendrites of cultured hippocampal neurons. We show that
transferrin receptors (TfRs) are constitutively internalized at optically static
clathrin-coated structures. These structures are slightly enriched near synapses
that represent preferential sites for the endocytosis of postsynaptic AMPA-type
receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of
AMPAR endocytosis events increases after the induction of NMDAR-dependent
chemical LTD, but the activity of perisynaptic endocytic zones is not
differentially regulated. We conclude that endocytosis is a highly dynamic and
stereotyped process that internalizes receptors in precisely localized endocytic
zones.
Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.