Single Nanoparticle Photothermal Tracking (SNaPT) of 5-nm gold beads in live cells
Biophysical Journal. 2006-12-01; 91(12): 4598-4604
DOI: 10.1529/biophysj.106.089771
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1. Biophys J. 2006 Dec 15;91(12):4598-604. Epub 2006 Sep 22.
Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live
cells.
Lasne D(1), Blab GA, Berciaud S, Heine M, Groc L, Choquet D, Cognet L, Lounis B.
Author information:
(1)Centre de Physique Moléculaire Optique et Hertzienne, CNRS (UMR 5798) and
Université Bordeaux 1, Talence Cedex, France.
Tracking individual nano-objects in live cells during arbitrary long times is a
ubiquitous need in modern biology. We present here a method for tracking
individual 5-nm gold nanoparticles on live cells. It relies on the photothermal
effect and the detection of the Laser Induced Scattering around a NanoAbsorber
(LISNA). The key point for recording trajectories at video rate is the use of a
triangulation procedure. The effectiveness of the method is tested against single
fluorescent molecule tracking in live COS7 cells on subsecond timescales. We
further demonstrate recordings for several minutes of AMPA receptors trajectories
on the plasma membrane of live neurons. Single Nanoparticle Photothermal Tracking
has the unique potential to record arbitrary long trajectory of membrane proteins
using nonfluorescent nanometer-sized labels.
DOI: 10.1529/biophysj.106.089771
PMCID: PMC1779909
PMID: 16997874 [Indexed for MEDLINE]