Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
Nat Commun. 2017-11-10; 8(1):
DOI: 10.1038/s41467-017-01752-5
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1. Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5.
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Martineau M(1)(2), Somasundaram A(3), Grimm JB(4), Gruber TD(4), Choquet
D(1)(2)(5), Taraska JW(6), Lavis LD(7), Perrais D(1)(2).
Author information:
(1)University of Bordeaux, F-33000, Bordeaux, France.
(2)Centre National de la Recherche Scientifique, Interdisciplinary Institute for
Neuroscience, UMR 5297, F-33000, Bordeaux, France.
(3)National Heart, Lung, and Blood Institute, US National Institutes of Health,
Bethesda, MD, 20892, USA.
(4)Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive,
Ashburn, VA, 20147, USA.
(5)Bordeaux Imaging Center, UMS 3420 CNRS, Université de Bordeaux, US 4 INSERM,
F-33000, Bordeaux, France.
(6)National Heart, Lung, and Blood Institute, US National Institutes of Health,
Bethesda, MD, 20892, USA. .
(7)Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive,
Ashburn, VA, 20147, USA. .
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and
endocytosis, but its performance has not been duplicated in red fluorescent
protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein
conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that
match the properties of SEP. Conjugation to genetically encoded self-labeling
tags or antibodies allows visualization of both exocytosis and endocytosis,
constituting new bright sensors for these key steps of synaptic transmission.
DOI: 10.1038/s41467-017-01752-5
PMCID: PMC5680258
PMID: 29123102